Bioassays for bomb-makers: proof of concept

被引：7

作者：

Bell, Suzanne C. ^{[1
]}

Gayton-Ely, Melissa ^{[1
]}

Nida, Corey M. ^{[1
]}

机构：

[1] W Virginia Univ, C Eugene Bennett Dept Chem, Morgantown, WV 26506 USA

来源：

ANALYTICAL AND BIOANALYTICAL CHEMISTRY | 2009年 / 395卷 / 02期

关键词：

Explosives; Biomarkers; Metabolism; LC/MS/MS; IONIZATION MASS-SPECTROMETRY; IN-VITRO; DRUG-METABOLISM; NITROAROMATIC COMPOUNDS; CHROMATOGRAPHIC METHODS; EXPLOSIVES; RDX; 2,4,6-TRINITROTOLUENE; TOXICOLOGY; TOXICITY;

D O I：

10.1007/s00216-009-2851-4

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Clandestine bomb-makers are exposed to significant amounts of explosives and allied materials. As with any ingested xenobiotic substance, these compounds are subject to biotransformation. As such, the potential exists that characteristic suites of biomarkers may be produced and deposited in matrices that can be exploited for forensic and investigative purposes. However, before such assays can be developed, foundational data must be gathered regarding the toxicokinetics, fate, and transport of the resulting biomarkers within the body and in matrices such as urine, hair, nails, sweat, feces, and saliva. This report presents an in vitro method for simulation of human metabolic transformations using human liver microsomes and an assay applicable to representative nitro-explosives. Control and metabolized samples of TNT, RDX, HMX, and tetryl were analyzed using high-performance liquid chromatography coupled to tandem mass spectrometry (LC/MS/MS) and biomarkers identified for each. The challenges associated with this method arise from solubility issues and limitations imposed by instrumentation, specifically, modes of ionization.

引用

页码：401 / 409

页数：9

共 56 条

[1] Use of liquid chromatography/tandem mass spectrometry to detect distinctive indicators of in situ RDX transformation in contaminated groundwater [J].

Beller, HR ;

Tiemeier, K .

ENVIRONMENTAL SCIENCE & TECHNOLOGY, 2002, 36 (09) :2060-2066

[2] Chemotaxis-mediated biodegradation of cyclic nitramine explosives RDX9 HMX, and CL-20 by Clostridium sp EDB2 [J].

Bhushan, B ;

Halasz, A ;

Thiboutot, S ;

Ampleman, G ;

Hawari, J .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2004, 316 (03) :816-821

[3] Hair as a biological indicator of drug use, drug abuse or chronic exposure to environmental toxicants [J].

Boumba, Vassiliki A. ;

Ziavrou, Kallirroe S. ;

Vougiouklakis, Theodore .

INTERNATIONAL JOURNAL OF TOXICOLOGY, 2006, 25 (03) :143-163

[4] An update on in vitro test methods in human hepatic drug biotransformation research: pros and cons [J].

Brandon, EFA ;

Raap, CD ;

Meijerman, I ;

Beijnen, JH ;

Schellens, JHM .

TOXICOLOGY AND APPLIED PHARMACOLOGY, 2003, 189 (03) :233-246

[5]

Chen HW, 2006, CHINESE J ANAL CHEM, V34, P839

[6] Assessment of Three Human in Vitro Systems in the Generation of Major Human Excretory and Circulating Metabolites [J].

Dalvie, Deepak ;

Obach, R. Scott ;

Kang, Ping ;

Prakash, Chandra ;

Loi, Cho-Ming ;

Hurst, Susan ;

Nedderman, Angus ;

Goulet, Lance ;

Smith, Evan ;

Bu, Hai-Zhi ;

Smith, Dennis A. .

CHEMICAL RESEARCH IN TOXICOLOGY, 2009, 22 (02) :357-368

[7]

de Oliveira CDR, 2007, CURR PHARM ANAL, V3, P95

[8] Adsorption of explosive molecules on human hair surfaces [J].

Efremenko, Irena ;

Zach, Raya ;

Zeiri, Yehuda .

JOURNAL OF PHYSICAL CHEMISTRY C, 2007, 111 (32) :11903-11911

[9] Methods and measurements for estimating human dermal uptake of volatile organic compounds and for deriving dermal permeability coefficients [J].

Fan, Victor S. ;

Savage, Russell E., Jr. ;

Buckley, Timothy J. .

TOXICOLOGY MECHANISMS AND METHODS, 2007, 17 (05) :295-304

[10] Nitramine anion fragmentation:: A mass spectrometric and ab initio study [J].

Florian, Jan ;

Gao, Lan ;

Zhukhovskyy, Vladimir ;

MacMillan, Denise K. ;

Chiarelli, M. Paul .

JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY, 2007, 18 (05) :835-841

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