Acute renal failure during sepsis: Potential role of cell cycle regulation

被引：89

作者：

Yang, Quan-hui ^{[1
]}

Liu, Da-wei ^{[1
]}

Long, Yun ^{[1
]}

Liu, Hong-zhong ^{[1
]}

Chai, Wen-zhao ^{[1
]}

Wang, Xiao-Ting ^{[1
]}

机构：

[1] Chinese Acad Med Sci, Peking Union Med Coll Hosp, Dept Crit Care Med, Beijing 100730, Peoples R China

来源：

JOURNAL OF INFECTION | 2009年 / 58卷 / 06期

基金：

中国博士后科学基金;

关键词：

Renal failure; Acute; Cyclin; Cyclin-dependent kinase; Cyclin-dependent kinase inhibitor; ACTIVATED PROTEIN-C; EPIDEMIOLOGY; APOPTOSIS; MORTALITY; FLOW;

D O I：

10.1016/j.jinf.2009.04.003

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

Objectives: This study aimed to evaluate cell cycle regulation in acute kidney injury after intraperitoneal sepsis in rats. Methods: Polymicrobial. sepsis was induced by cecal ligation and puncture (CLP) in rats. At 0, 6, 12, 24, 48, and 72 h after CLP, serum creatinine was evaluated. DNA content of isolated kidney cells was analyzed using flow cytometer. Furthermore, the expression of p21, p53, cyclin D1, cyclin E, CDK2, CDK4 and P-pRb was also measured by western blot. Results: After sepsis-induced by CLP, kidney injury of rat was associated with G1 cell cycle arrest, however, recovery of renal function related to cell cycle progression 48 h after CLP. Results also showed that the upregulation of p53 and p21 was correlated with G1 cell arrest in 48 h after CLP. Nevertheless, upregulation of cyclin D1/CDK4 and cyclin E/CDK2 induced pRb phosphorylation, which resulted in the G1/S transition 48 h after CLP. Conclusion: The data suggest that G1 cell cycle arrest may play a role in the initiation of kidney injury, whereas, through regulating cell cycle, p53, p21, CDKs, cyclins and P-pRb may be involved in the injury or recovery of renal function after intraperitoneal sepsis. (C) 2009 The British Infection Society. Published by Elsevier Ltd. All rights reserved.

引用

页码：459 / 464

页数：6

共 35 条

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