Design, synthesis and evaluation of new alkylated pyrimidine derivatives as antibacterial agents

In order to assess their antibacterial capabilities, a unique set of alkylated pyrimidine derivatives were designed and synthesized. The intended compounds were produced in good yields, structural elucidation of the synthesised molecules was carried out using 13C NMR, 1H NMR, LCMS, elemental analysis and IR spectroscopy. The purity of the synthesied molecules were analysed by HPLC and purity for some compounds was found to be >99 % a/a and few were >95 % a/a. Among the synthesised compounds, 5a, 5b, 5e,5j and 5n have potent activity as antibacterial agents and significantly inhibited bacterial growth of Xanthomonas campestris pv. Campestris, Pseudomonas fluorescens and Bacillus subtilis. The recorded inhibition for Xanthomonas campestris pv. Campestris by recording a percent inhibition of 63.90, 65.76,67.12,67.46 and 66.78. For Pseudomonas fluorescens by recording a percent inhibition 70.61, 71.33,69.89,73.12, and 70.97 and for Bacillus subtilis by recording a percent inhibition of 51.02, 51.39,57.49,56.56, and 58.41 respectively.