Advances in the field of single-particle cryo-electron microscopy over the last decade

被引：106

作者：

Frank, Joachim ^{[1
,2
]}

机构：

[1] Columbia Univ, Dept Biochem & Mol Biophys, 630 W 168th St, New York, NY 10027 USA

[2] Columbia Univ, Dept Biol Sci, New York, NY 10027 USA

来源：

NATURE PROTOCOLS | 2017年 / 12卷 / 02期

基金：

美国国家卫生研究院;

关键词：

ESCHERICHIA-COLI RIBOSOME; CRYO-EM STRUCTURE; ELECTRON CRYOMICROSCOPY; MACROMOLECULAR COMPLEXES; RESOLUTION; MODEL; DYNAMICS; SUBUNIT; RNA;

D O I：

10.1038/nprot.2017.004

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

In single-particle cryo-electron microscopy (cryo-EM), molecules suspended in a thin aqueous layer are rapidly frozen and imaged at cryogenic temperature in the transmission electron microscope. From the random projection views, a three-dimensional image is reconstructed, enabling the structure of the molecule to be obtained. In this article I discuss technological progress over the past decade, which has, in my own field of study, culminated in the determination of ribosome structure at 2. resolutn. I also discuss likely future improvements in methodology.

引用

页码：209 / 212

页数：4

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