Biological Sulfur-Oxidizing Potential of Primary and Biological Sludge in a Tannery Wastewater Treatment Plant

被引:9
作者
Giordano, Cesira [1 ]
Spennati, Francesco [2 ]
Melone, Anna [1 ]
Petroni, Giulio [1 ]
Verni, Franco [1 ]
Munz, Giulio [2 ]
Mori, Gualtiero [3 ]
Vannini, Claudia [1 ]
机构
[1] Univ Pisa, Dept Biol, Protistol Zool Unit, I-56126 Pisa, Italy
[2] Univ Florence, Dept Civil & Environm Engn, I-50139 Florence, Italy
[3] CER2CO Ctr Ric Reflui Conciari, I-56020 Pisa, Italy
关键词
Hydrosulfuric acid; Sulfhydric acid; 16S rRNA; Sulfur; Sulfide; Halothiobacillus; HYDROGEN-SULFIDE; AUTOTROPHIC DENITRIFICATION; BACTERIA; OXIDATION; REMOVAL; IDENTIFICATION; BIOREACTOR; PERFORMANCE; DIVERSITY; WASTEWATERS;
D O I
10.1007/s11270-015-2656-3
中图分类号
X [环境科学、安全科学];
学科分类号
08 ; 0830 ;
摘要
Hydrogen sulfide (H2S) is one of the major tannery wastewater pollutants. Up today, chemical scrubbing is the most established technology for H2S removal in wastewater treatment plants. However, this procedure increases both the operating costs and the carbon footprint of the treatment. On the other hand, biological treatment is an emerging and sustainable technology for air pollution control. Our study focuses on the biological sulfur-oxidizing potential of autochthonous sludge of a plant treating tannery wastewater, located in Santa Croce (Tuscany, Italy). We propose a multidisciplinary approach to investigate two aspects: first, the composition of the microbial community both in the biological sludge and in the primary sludge and second, the sulfur-oxidizing potential of these native matrices. In order to do that, biological and primary sludge were used as inoculum in a reactor fed with sulfides. Then, traditional cultivation techniques were combined with several molecular approaches for analyzing the microbial community structure with a special focus on sulfur-oxidizing bacteria (SOB). Our study demonstrated that the use of different techniques was fundamental in order to detect the largest number of sulfur-oxidizing bacterial components; in particular, the detection of less represented components was guaranteed only by the performed multidisciplinary approach. Our study demonstrated the optimal performances of the reactor in selecting a sulfur-oxidizing biomass from autochthonous matrices. In addition, the importance of the primary sludge as inoculum for sulfur-oxidizing reactors was proved.
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页数:13
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共 56 条
[1]   Gapped BLAST and PSI-BLAST: a new generation of protein database search programs [J].
Altschul, SF ;
Madden, TL ;
Schaffer, AA ;
Zhang, JH ;
Zhang, Z ;
Miller, W ;
Lipman, DJ .
NUCLEIC ACIDS RESEARCH, 1997, 25 (17) :3389-3402
[2]   FLUORESCENT-OLIGONUCLEOTIDE PROBING OF WHOLE CELLS FOR DETERMINATIVE, PHYLOGENETIC, AND ENVIRONMENTAL-STUDIES IN MICROBIOLOGY [J].
AMANN, RI ;
KRUMHOLZ, L ;
STAHL, DA .
JOURNAL OF BACTERIOLOGY, 1990, 172 (02) :762-770
[3]   SPECIES DIVERSITY - BENTHONIC FORAMINIFERA IN WESTERN NORTH ATLANTIC [J].
BUZAS, MA ;
GIBSON, TG .
SCIENCE, 1969, 163 (3862) :72-&
[4]   Removal of sulfur inorganic compounds by a biofilm of sulfate reducing and sulfide oxidizing bacteria in a down-flow fluidized bed reactor [J].
Celis-Garcia, Lourdes B. ;
Gonzalez-Blanco, Gehovana ;
Meraz, Monica .
JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY, 2008, 83 (03) :260-268
[5]  
Cha JM, 1999, PROCESS BIOCHEM, V34, P659
[6]   Bacterial Community Shift in Two Sectors of a Tannery Plant and its Cr (VI) Removing Potential [J].
Chen, Jian ;
Tang, Yue-Qin ;
Wu, Xiao-Lei .
GEOMICROBIOLOGY JOURNAL, 2012, 29 (03) :226-235
[7]   Hydrogen sulfide gas treatment by a chemical-biological process: Chemical absorption and biological oxidation steps [J].
Chung, YC ;
Ho, KL ;
Tseng, CP .
JOURNAL OF ENVIRONMENTAL SCIENCE AND HEALTH PART B-PESTICIDES FOOD CONTAMINANTS AND AGRICULTURAL WASTES, 2003, 38 (05) :663-679
[8]   Biological waste air treatment in biotrickling filters [J].
Cox, HHJ ;
Deshusses, MA .
CURRENT OPINION IN BIOTECHNOLOGY, 1998, 9 (03) :256-262
[9]   Antibiotic-resistant. soil bacteria in transigenic plant fields [J].
Demaneche, Sandrine ;
Sanguin, Herve ;
Pote, John ;
Navarro, Elisabeth ;
Bernillon, Dominique ;
Mavingui, Patrick ;
Wildi, Walter ;
Vogel, Timothy M. ;
Simonet, Pascal .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2008, 105 (10) :3957-3962
[10]   Microbial Community Structure and Diversity in an Integrated System of Anaerobic-Aerobic Reactors and a Constructed Wetland for the Treatment of Tannery Wastewater in Modjo, Ethiopia [J].
Desta, Adey Feleke ;
Assefa, Fassil ;
Leta, Seyoum ;
Stomeo, Francesca ;
Wamalwa, Mark ;
Njahira, Moses ;
Appolinaire, Djikeng .
PLOS ONE, 2014, 9 (12)