Postmortem interval determination using 18S-rRNA and microRNA

被引：37

作者：

Li Wen-Can ^{[1
,5
]}

Ma Kai-Jun ^{[2
]}

Lv Ye-Hui ^{[1
]}

Zhang Ping ^{[1
]}

Pan Hui ^{[1
]}

Zhang Heng ^{[1
]}

Wang Hui-Jun ^{[3
]}

Ma Duan ^{[4
]}

Chen Long ^{[1
]}

机构：

[1] Fudan Univ, Forens Dept, Shanghai Med Coll, Shanghai 200032, Peoples R China

[2] Shanghai Publ Secur Bur, Forens Lab, Criminal Sci & Technol Inst, Shanghai 200032, Peoples R China

[3] Fudan Univ, Childrens Hosp, Shanghai 201102, Peoples R China

[4] Fudan Univ, Key Lab Mol Med, Minist Educ, Dept Biochem & Mol Biol, Shanghai 200032, Peoples R China

[5] Shanghai City Publ Secur Bur, Shanghai 200032, Peoples R China

来源：

SCIENCE & JUSTICE | 2014年 / 54卷 / 04期

关键词：

Postmortem interval (PMI); 18S-rRNA; microRNA; Reverse transcription real-time quantitative polymerase chain reaction (RT-qPCR); REAL-TIME-PCR; MESSENGER-RNA; STABILITY; DEGRADATION; EXPRESSION; PROTEIN;

D O I：

10.1016/j.scijus.2014.03.001

中图分类号：

DF [法律]; D9 [法律]; R [医药、卫生];

学科分类号：

0301 ; 10 ;

摘要：

The importance of determining postmortem interval (PMI) is crucial to criminal, civil and forensic cases. The precise estimation of PMI is a critical step in many death investigations. A technique exploiting the level of RNA, 18S rRNA and microRNA to estimate PMI was investigated. 18S-rRNA is a main ribosomal RNA presented as part of the ribosomal protein complex, while microRNA is a class of small non-coding single-stranded RNA, only 21-25 nucleotides, which has a strong conservation between different species. In this study, heart tissues were removed from adult rats at various postmortem intervals. An efficient extraction and detection protocol to analyze the level of 18S-rRNA and microRNA in postmortem tissue was carried out. The process consists of total RNA extraction, transcription and visualization by quantitative real time PCR. The result indicates a characteristic parabola relationship between postmortem period and Ct values for 18S-rRNA in dead rat hearts. The result indicates that the degradation pattern of tissue 18S-rRNA and microRNA is useful in the determination of the postmortem interval within seven days. (C) 2014 The Chartered Society of Forensic Sciences. Published by Elsevier Ireland Ltd. All rights reserved.

引用

页码：307 / 310

页数：4

共 26 条

[1] A method for determining the age of a bloodstain [J].

Anderson, S ;

Howard, B ;

Hobbs, GR ;

Bishop, CP .

FORENSIC SCIENCE INTERNATIONAL, 2005, 148 (01) :37-45

[2]

[Anonymous], FA YI XUE ZA ZHI V0

[3]

[Anonymous], 1995, POSTMORTEM CHANGES T

[4]

Bauer Martin, 2003, Leg Med (Tokyo), V5, P220, DOI 10.1016/j.legalmed.2003.08.001

[5] The MIQE Guidelines: Minimum Information for Publication of Quantitative Real-Time PCR Experiments [J].

Bustin, Stephen A. ;

Benes, Vladimir ;

Garson, Jeremy A. ;

Hellemans, Jan ;

Huggett, Jim ;

Kubista, Mikael ;

Mueller, Reinhold ;

Nolan, Tania ;

Pfaffl, Michael W. ;

Shipley, Gregory L. ;

Vandesompele, Jo ;

Wittwer, Carl T. .

CLINICAL CHEMISTRY, 2009, 55 (04) :611-622

[6] Is flow cytometric evaluation of DNA degradation a reliable method to investigate the early postmortem period? [J].

Di Nunno, NR ;

Costantinides, F ;

Bernasconi, P ;

Bottin, C ;

Melato, M .

AMERICAN JOURNAL OF FORENSIC MEDICINE AND PATHOLOGY, 1998, 19 (01) :50-53

[7] STABILITY OF PROTEIN AND MESSENGER-RNA IN HUMAN POSTMORTEM LIVER ANALYSIS BY TWO-DIMENSIONAL GEL-ELECTROPHORESIS [J].

FINGER, JM ;

MERCER, JFB ;

COTTON, RGH ;

DANKS, DM .

CLINICA CHIMICA ACTA, 1987, 170 (2-3) :209-218

[8] Endogenous control genes in complex vascular tissue samples [J].

Folkersen, Lasse ;

Kurtovic, Sanela ;

Razuvaev, Anton ;

Agardh, Hanna E. ;

Gabrielsen, Anders ;

Paulsson-Berne, Gabrielle .

BMC GENOMICS, 2009, 10

[9]

He L., 2004, NAT REV GENET, V5, P522

[10]

HUMPHREYS-BEHER M G, 1986, Biotechnology and Applied Biochemistry, V8, P392

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