Microfluidics for cell-based high throughput screening platformsd-A review

被引：200

作者：

Du, Guansheng ^{[1
,2
]}

Fang, Qun ^{[1
]}

den Toonder, Jaap M. J. ^{[2
]}

机构：

[1] Zhejiang Univ, Dept Chem, Inst Microanalyt Syst, Hangzhou 310058, Zhejiang, Peoples R China

[2] Eindhoven Univ Technol, Inst Complex Mol Syst, Mat Technol Inst, Dept Mech Engn, NL-5600 MB Eindhoven, Netherlands

来源：

ANALYTICA CHIMICA ACTA | 2016年 / 903卷

基金：

中国国家自然科学基金;

关键词：

High throughput screening; Cell-based microfluidics; Drug discovery; RESEARCH-AND-DEVELOPMENT; LARGE-SCALE INTEGRATION; DRUG DISCOVERY; MODIFYING POLY(DIMETHYLSILOXANE); CONVENIENT METHOD; SOFT LITHOGRAPHY; CULTURE SYSTEM; DROPLET ARRAY; CHIP; DEVICES;

D O I：

10.1016/j.aca.2015.11.023

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

In the last decades, the basic techniques of microfluidics for the study of cells such as cell culture, cell separation, and cell lysis, have been well developed. Based on cell handling techniques, microfluidics has been widely applied in the field of PCR (Polymerase Chain Reaction), immunoassays, organ-on-chip, stem cell research, and analysis and identification of circulating tumor cells. As a major step in drug discovery, high-throughput screening allows rapid analysis of thousands of chemical, biochemical, genetic or pharmacological tests in parallel. In this review, we summarize the application of microfluidics in cell-based high throughput screening. The screening methods mentioned in this paper include approaches using the perfusion flow mode, the droplet mode, and the microarray mode. We also discuss the future development of microfluidic based high throughput screening platform for drug discovery. (C) 2015 Elsevier B.V. All rights reserved.

引用

页码：36 / 50

页数：15

共 80 条

[1] Cell culture: Biology's new dimension [J].

Abbott, A .

NATURE, 2003, 424 (6951) :870-872

[2] Drug repositioning: Identifying and developing new uses for existing drugs [J].

Ashburn, TT ;

Thor, KB .

NATURE REVIEWS DRUG DISCOVERY, 2004, 3 (08) :673-683

[3] Millifluidic droplet analyser for microbiology [J].

Baraban, Larysa ;

Bertholle, Fabien ;

Salverda, Merijn L. M. ;

Bremond, Nicolas ;

Panizza, Pascal ;

Baudry, Jean ;

de Visser, J. Arjan G. M. ;

Bibette, Jerome .

LAB ON A CHIP, 2011, 11 (23) :4057-4062

[4] Digital microfluidics for cell-based assays [J].

Barbulovic-Nad, Irena ;

Yang, Hao ;

Park, Philip S. ;

Wheeler, Aaron R. .

LAB ON A CHIP, 2008, 8 (04) :519-526

[5] Organs on Chips 2013 [J].

Beebe, David J. ;

Ingber, Donald E. ;

den Toonder, Jaap .

LAB ON A CHIP, 2013, 13 (18) :3447-3448

[6] Physics and applications of microfluidics in biology [J].

Beebe, DJ ;

Mensing, GA ;

Walker, GM .

ANNUAL REVIEW OF BIOMEDICAL ENGINEERING, 2002, 4 :261-286

[7] Engineering cellular microenvironments to cell-based drug testing improve [J].

Bhadriraju, K ;

Chen, CS .

DRUG DISCOVERY TODAY, 2002, 7 (11) :612-620

[8] Detecting bacteria and determining their susceptibility to antibiotics by stochastic confinement in nanoliter droplets using plug-based microfluidics [J].

Boedicker, James Q. ;

Li, Liang ;

Kline, Timothy R. ;

Ismagilov, Rustem F. .

LAB ON A CHIP, 2008, 8 (08) :1265-1272

[9] A digital microfluidic method for multiplexed cell-based apoptosis assays [J].

Bogojevic, Dario ;

Chamberlain, M. Dean ;

Barbulovic-Nad, Irena ;

Wheeler, Aaron R. .

LAB ON A CHIP, 2012, 12 (03) :627-634

[10] Droplet microfluidic technology for single-cell high-throughput screening [J].

Brouzes, Eric ;

Medkova, Martina ;

Savenelli, Neal ;

Marran, Dave ;

Twardowski, Mariusz ;

Hutchison, J. Brian ;

Rothberg, Jonathan M. ;

Link, Darren R. ;

Perrimon, Norbert ;

Samuels, Michael L. .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2009, 106 (34) :14195-14200

← 1 2 3 4 5 6 7 8 →