Role of Cold Shock Proteins in Growth of Listeria monocytogenes under Cold and Osmotic Stress Conditions

被引:149
作者
Schmid, Barbara [1 ]
Klumpp, Jochen [2 ]
Raimann, Eveline [1 ]
Loessner, Martin J. [2 ]
Stephan, Roger [1 ]
Tasara, Taurai [1 ]
机构
[1] Univ Zurich, Vetsuisse Fac, Inst Food Safety & Hyg, CH-8057 Zurich, Switzerland
[2] ETH, Inst Food Sci & Nutr, CH-8092 Zurich, Switzerland
关键词
ESCHERICHIA-COLI; BACILLUS-SUBTILIS; CSPA FAMILY; GENE DUPLICATION; LOW-TEMPERATURE; MESSENGER-RNA; EXPRESSION; MEMBER; PHASE; CELLS;
D O I
10.1128/AEM.02154-08
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
The gram-positive bacterium Listeria monocytogenes is a food-borne pathogen of both public health and food safety significance. It possesses three small, highly homologous protein members of the cold shock protein (Csp) family. We used gene expression analysis and a set of mutants with single, double, and triple deletions of the csp genes to evaluate the roles of CspA, CspB, and CspD in the cold and osmotic (NaCl) stress adaptation responses of L. monocytogenes. All three Csps are dispensable for growth at optimal temperature (37 degrees C). These proteins are, however, required for efficient cold and osmotic stress tolerance of this bacterium. The hierarchies of their functional importance differ, depending on the environmental stress conditions: CspA > CspD > CspB in response to cold stress versus CspD > CspA/CspB in response to NaCl salt osmotic stress. The fact that Csps are promoting L. monocytogenes adaptation against both cold and NaCl stress has significant implications in view of practical food microbial control measures. The combined or sequential exposure of L. monocytogenes cells to these two stresses in food environments might inadvertently induce cross-protection responses.
引用
收藏
页码:1621 / 1627
页数:7
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