Solid Lipid Nanoparticles Loaded with Fluorescent-labelled Cyclosporine A: Anti-Inflammatory Activity In Vitro

被引：4

作者：

Gallarate, Marina ^{[1
]}

Serpe, Loredana ^{[1
]}

Foglietta, Federica ^{[1
]}

Zara, Gian Paolo ^{[1
]}

Giordano, Susanna ^{[1
]}

Peira, Elena ^{[1
]}

Chirio, Daniela ^{[1
]}

Battaglia, Luigi ^{[1
]}

机构：

[1] Univ Turin, Dipartimento Sci & Tecnol Farm, Turin, Italy

来源：

PROTEIN AND PEPTIDE LETTERS | 2014年 / 21卷 / 11期

关键词：

Coacervation; cyclosporine A; cytokines; FMOC; inflammation; solid lipid nanoparticles; DELIVERY; SLN;

D O I：

10.2174/0929866521666140806164410

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

FMOC-isocyclosporine A, a fluorescent labeled cyclosporine A, was encapsulated in solid lipid nanoparticles (SLN) prepared by the coacervation technique, and its anti-inflammatory activity was evaluated. The anti-inflammatory activity of the fluorescent labelled molecule, measured as inhibition of TNF-alpha secretion, is similar to the native one. SLN were compared to commercial formulations, through measurement of cytokine release and drug uptake in rat peripheral blood mononuclear cells. Drug-loaded SLN inhibit TNF-alpha secretion in a lower extent than commercial formulations, probably due to a lower uptake by the cells, but the increase of IL-10 secretion caused by the lipid matrix itself makes this formulation interesting for its anti-inflammatory activity.

引用

页码：1157 / 1162

页数：6

共 14 条

[1]

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[2] Nanoparticulate Delivery Can Improve Peroral Bioavailability of Cyclosporine and Match Neoral Cmax Sparing the Kidney from Damage [J].