Cloning and Characterization of the a†6 Polyunsaturated Fatty Acid Elongase from the Green Microalga Parietochloris incisa

被引：22

作者：

Iskandarov, Umidjon ^{[1
]}

Khozin-Goldberg, Inna ^{[1
]}

Ofir, Rivka ^{[2
]}

Cohen, Zvi ^{[1
]}

机构：

[1] Ben Gurion Univ Negev, Jacob Blaustein Inst Desert Res, Microalgal Biotechnol Lab, IL-84990 Midreshet Ben Gurion, Israel

[2] Ben Gurion Univ Negev, Dead Sea & Arava Sci Ctr, IL-84105 Beer Sheva, Israel

来源：

LIPIDS | 2009年 / 44卷 / 06期

关键词：

Arachidonic acid; Fatty acid elongation; Nitrogen starvation; Parietochloris incisa; Subtractive hybridization; VLC-PUFA; LIVERWORT MARCHANTIA-POLYMORPHA; RATE-LIMITING STEP; ARACHIDONIC-ACID; FUNCTIONAL-CHARACTERIZATION; EICOSAPENTAENOIC ACID; DOCOSAHEXAENOIC ACID; MORTIERELLA-ALPINA; MOLECULAR EVIDENCE; BIOSYNTHESIS; IDENTIFICATION;

D O I：

10.1007/s11745-009-3301-y

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The very-long-chain polyunsaturated fatty acid (VLC-PUFA), arachidonic acid (ARA, 20:4 omega-6) is a component of neuron tissues such as brain and retina cells and a primary substrate for the biosynthesis of biologically active eicosanoids. The green freshwater microalga Parietochloris incisa (Trebouxiophyceae) has been shown to accumulate an extraordinary high content of ARA-rich triacylglycerols. It was thus interesting to characterize the genes involved in lipid biosynthesis in this alga. We report here the identification of a cDNA encoding for a P. incisa PUFA elongase (PiELO1) and demonstrate that the expression of PiELO1 in yeast Saccharomyces cereviseae confers its elongase activity on C18 a dagger 6 PUFA. Phylogenetic analysis indicated that PiELO1 is highly similar to functionally characterized a dagger 6 PUFA elongase genes from other green algae and lower plants. Quantitative real-time PCR expression studies showed that PiELO1 is upregulated under nitrogen starvation, the condition triggering and enhancing storage oil and ARA accumulation in P. incisa.

引用

页码：545 / 554

页数：10

共 42 条

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