Recent Advance in Chemiluminescence Assay and Its Biochemical Applications

Chemiluminescence (CL) assay is able to measure the optical signal emitted from the CL reagents as a result of the transition from the excited state back to the ground state. CL assay has significant advantages such as no external light source, high sensitivity, convenient operation, rapid analysis and easy automation, and has wide applications in clinical test, drug analysis and environmental monitoring. Recently, the introduction of nanomaterials, biochip and microfluidic techniques promote the development of CL assay. In this review, we summarize the recent progress in CL assay with the integration of high performance liquid chromatography, capillary electrophoresis, quantum dots, microfluidic chips, microarrays, rolling circle amplification, isothermal exponential amplification, and two. stage isothermal amplification for the detection of DNA, small biological molecules, enzymes, proteins and metal ions. We also give a summary of its future directions and highlight its potential applications.