Overexpression of miR-493-3p suppresses ovarian cancer cell proliferation, migration and invasion through downregulating DPY30

Accumulating evidence has verified that the aberrant expression level of miR-493-3p is often associated with the occurrence of numerous cancers. Nevertheless, the expression level and effect of this microRNA in ovarian cancer (OC) remain largely unclear. Therefore, the molecular function of miR-493-3p in OC progression was systematically investigated in this study. The expression of miR-493-3p and DPY30 was assessed by qRT-PCR. The protein expression level of DPY30 in cell lines was further assessed by western blot. Cell viability was respectively examined in vitro functional experiments including CCK-8 assay, EdU assay, wound healing assay, colony formation and apoptosis assays as well as the scratch test and transwell assay. Bioinformatics analysis and luciferase reporter assays were performed to predict and clarity of the correlation between miR-493-3p and DPY30. The expression of miR-493-3p was significantly reduced in OC tissues and cells. Functional experimental results showed that miR-493-3p suppressed cellular proliferation, migration, invasion, but promoted apoptosis in OC cells. Mechanistically, we also confirmed that DPY30 could be directly targeted by miR-493-3p based on bioinformatics and dual-luciferase reporter analysis. Rescue experiments results indicated that the inhibitory effect of miR-493-3p on cellular proliferation, migration and invasion and the promotive effect of miR-493-3p on apoptosis was abolished by DPY30 overexpression. Our findings demonstrated the antitumor effect of miR-493-3p through targeting DPY30 in ovarian cancer, indicating that miR-493-3p might represent a promising target for ovarian cancer diagnosis and treatment. (c) 2022 Published by Elsevier B.V. on behalf of Society for Biology of Reproduction & the Institute of Animal Reproduction and Food Research of Polish Academy of Sciences in Olsztyn.