New carboxysilane-coated iron oxide nanoparticles for nonspecific cell labelling

被引:22
作者
Bridot, Jean-Luc [1 ]
Stanicki, Dimitri [1 ]
Laurent, Sophie [1 ]
Boutry, Sebastien [2 ]
Gossuin, Yves [3 ]
Leclere, Philippe [4 ]
Lazzaroni, Roberto [4 ]
Vander Elst, Luce [1 ]
Muller, Robert N. [1 ,2 ]
机构
[1] UMONS, Dept Gen Organ & Biomed Chem, NMR & Mol Imaging Lab, B-7000 Mons, Belgium
[2] Ctr Microscopy & Mol Imaging, B-6041 Charleroi, Belgium
[3] UMONS, Phys Biol Lab, B-7000 Mons, Belgium
[4] UMONS, Ctr Innovat & Res Mat & Polymers, Lab Chem Novel Mat, B-7000 Mons, Belgium
关键词
nanoparticles; iron oxide; cell labelling; ferrofluids; silane; colloidal stability; contrast agent; MR CONTRAST AGENTS; IN-VIVO MRI; MAGNETIC NANOPARTICLES; OPTIMIZATION; TRACKING; RELAXIVITY; SURFACE; STABILIZATION; FERUCARBOTRAN; FERUMOXIDES;
D O I
10.1002/cmmi.1552
中图分类号
R8 [特种医学]; R445 [影像诊断学];
学科分类号
1002 ; 100207 ; 1009 ;
摘要
Magnetic resonance imaging (MRI) offers the possibility of tracking cells labelled with a contrast agent and evaluating the progress of cell therapies. This requires efficient cell labelling with contrast agents. A basic incubation of cells with iron oxide nanoparticles (NPs) is a common method. This study reports the synthesis at the gram scale of iron oxide nanoparticles as MRI T-2 contrast agents for cell labelling. These NPs are based on small iron oxide cores coated with a thin polysiloxane shell presenting carboxylic acid functions. The iron oxide cores produced have been characterized by transmission electron microscopy, X-ray diffraction, -potential, infrared, photon correlation spectroscopy, atomic force microscopy, magnetometry and relaxometric measurements. These measurements confirmed the expected surface modification by carboxysilane. Carboxylic groups created electrostatic repulsion between NPs when they are deprotonated. Therefore, highly concentrated aqueous solutions of carboxysilane coated iron oxide NPs can be obtained, up to 70% (w/w). These NPs could be used for cell labelling owing to their aggregation and re-dispersion properties. NPs precipitated in Dulbecco's modified Eagle medium induced a rapid association with 3T6 fibroblast cells and could easily be re-dispersed in phosphate buffer saline solution to obtain properly labelled cells. Copyright (c) 2013 John Wiley & Sons, Ltd.
引用
收藏
页码:466 / 474
页数:9
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