Expression analysis of Escherichia coli lacZ reporter gene in transgenic mice

被引：6

作者：

Takahashi, E

Miyamoto, A

Kajiwara, N

Furuya, K

Yanai-Taniguchi, K

Sugiyama, F

Yagami, K

机构：

[1] Eisai & Co Ltd, Tsukuba Res Labs, Tsukuba, Ibaraki 3002635, Japan

[2] Univ Tsukuba, Lab Anim Res Ctr, Tsukuba, Ibaraki 3058527, Japan

来源：

BRAIN RESEARCH PROTOCOLS | 2000年 / 5卷 / 02期

关键词：

lacZ; transcriptional regulation; cis-regulatory element; transgenic mice;

D O I：

10.1016/S1385-299X(00)00009-X

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

To define a gene expression mechanism, it is often advantageous to use a reporter gene and transgenic mouse. The lacZ reporter gene is particularly useful for studies of the cis-regulatory element for tissue-specific expression in transgenic mice because of the ease of the enzyme assay and visualization on sections. In this report, we describe our method for examining the cis-regulatory element in transgenic mice, including choice of the lacZ gene, generation of transgenic mice, and analysis of beta-galactosidase activity. (C) 2000 Elsevier Science B.V. All rights reserved.

引用

页码：159 / 166

页数：8

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