In vivo combined virtual histology and vascular imaging with dual-wavelength photoacoustic remote sensing microscopy

Histological evaluation of tissues is currently a lengthy process that typically precludes intraoperative margin assessment. While numerous approaches have aimed to address the need for intraoperative virtual histology, none have yet proved sufficiently efficacious. We demonstrate the use of a newall-optical imaging modality, photoacoustic remote sensing (PARS), capable of virtual histopathological imaging, while simultaneously providing visualization of microvasculature in both freshly resected tissues and live animal subjects. We demonstrate high resolutions of 0.44 mu m and 1.2 mu m for 266-nm and 532-nm excitation wavelengths, respectively, as well as the characterization of maximum permissible exposure limits for both excitation wavelengths. (c) 2020 Optical Society of America under the terms of the OSA Open Access Publishing Agreement