Is ITS-2 rDNA suitable marker for genetic characterization of Sarcoptes mites from different wild animals in different geographic areas?

被引：49

作者：

Alasaad, S. ^{[2
,5
]}

Soglia, D. ^{[5
]}

Spalenza, V. ^{[5
]}

Maione, S. ^{[5
]}

Soriguer, R. C. ^{[3
]}

Perez, J. M. ^{[2
]}

Rasero, R. ^{[5
]}

Degiorgis, M. P. Ryser ^{[4
]}

Nimmervoll, H. ^{[4
]}

Zhu, X. Q. ^{[1
]}

Rossi, L. ^{[5
]}

机构：

[1] S China Agr Univ, Coll Vet Med, Guangzhou 510642, Guangdong, Peoples R China

[2] Univ Jaen, Dept Biol Anim Biol Vegetal & Ecol, E-23071 Jaen, Spain

[3] CSIC, Estac Biol Donana, E-41013 Seville, Spain

[4] Vetsuisse Fac Berne, Inst Anim Pathol, Ctr Fish & Wildlife Hlth, Bern, Switzerland

[5] Univ Turin, Dipartimento Prod Anim Epidemiol & Ecol, I-10095 Grugliasco, Italy

来源：

VETERINARY PARASITOLOGY | 2009年 / 159卷 / 02期

关键词：

Europe; Genetic characterization; Genotypes; Ribosomal DNA (rDNA); Second internal transcribed spacer (ITS-2); Sarcoptes scabiei; Wildlife; DNA-SEQUENCE; ACARI; MANGE; MITOCHONDRIAL; SCABIEI; HOSTS; TETRANYCHIDAE; EPIDEMIOLOGY; DIVERSITY;

D O I：

10.1016/j.vetpar.2008.10.001

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

The present Study examined the relationship among individual Sarcoptes scabiei mites from 13 wild mammalian populations belonging to nine species in four European Countries using the second internal transcribed spacer (ITS-2) Of nuclear ribosomal DNA (rDNA) as genetic marker. The ITS-2 plus primer flanking 5.8S and 28S rDNA (ITS-2+) was amplified from individual mites by polymerase chain reaction (PCR) and the amplicons were sequenced directly. A total of 148 ITS-2+ sequences of 404 bp ill length Were obtained and 67 variable sites were identified (16.59%). UPGMA analyses did not show any geographical or host-specific clustering, and a similar outcome was obtained using population pairwise Fst statistics. These results demonstrated that ITS-2 rDNA does not appear to be suitable for examining genetic diversity among mite populations. (C) 2008 Elsevier B.V. All rights reserved.

引用

页码：181 / 185

页数：5

共 32 条

[1] Skin-scale genetic structure of Sarcoptes scabiei populations from individual hosts: empirical evidence from Iberian ibex-derived mites [J].