Associations of genome-wide cell-free DNA fragmentation profiles with blood biochemical and hematological parameters in healthy individuals

被引：4

作者：

Bao, Hua ^{[1
]}

Chen, Xiaoxi ^{[1
]}

Xiao, Qian ^{[2
]}

Yang, Shanshan ^{[1
]}

Wu, Shuyu ^{[1
]}

Wang, Xiaonan ^{[1
]}

Wu, Xue ^{[1
]}

Ding, Kefeng ^{[2
,3
,5
]}

Shao, Yang ^{[1
,4
,6
]}

机构：

[1] Nanjing Geneseeq Technol Inc, Geneseeq Res Inst, Nanjing, Jiangsu, Peoples R China

[2] Zhejiang Univ Sch Med, Affiliated Hosp 2, Dept Colorectal Surg & Oncol, Key Lab Canc Prevent & Intervent,Minist Educ, Hangzhou, Zhejiang, Peoples R China

[3] Zhejiang Univ, Canc Ctr, Hangzhou, Zhejiang, Peoples R China

[4] Nanjing Med Univ, Sch Publ Hlth, Nanjing, Peoples R China

[5] Zhejiang Univ Sch Med, Affiliated Hosp 2, Dept Colorectal Surg & Oncol, Key Lab Canc Prevent & Intervent,Minist Educ, 300 Yuanju Rd, Hangzhou 310009, Zhejiang, Peoples R China

[6] Nanjing Geneseeq Technol Inc, 128 Huakang Rd, Nanjing 210000, Jiangsu, Peoples R China

来源：

GENOMICS | 2022年 / 114卷 / 06期

基金：

中国国家自然科学基金;

关键词：

Cell-free DNA; Fragmentomics; Hematology; Biochemical parameters; Liver enzyme; PLASMA DNA; SEQUENCE; DISEASE; CANCER; ORIGIN; SEX;

D O I：

10.1016/j.ygeno.2022.110504

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Cell-free DNA (cfDNA), as a non-invasive approach, has been introduced in a wide range of applications, including cancer diagnosis/ monitoring, prenatal testing, and transplantation monitoring. Yet, studies of cfDNA fragmentomics in physiological conditions are lacking. In this study, we aim to explore the correlation of frag-mentation patterns of cfDNA with blood biochemical and hematological parameters in healthy individuals. We addressed the impact of physiological variables and abnormal blood biochemical and hematological parameters on cfDNA fragment size distribution. We also figured and validated that hematological inflammation markers, including leukocyte, lymphocyte, neutrophil, and platelet distribution width as well as aspartate transaminase levels were significantly correlated with the genome-wide cfDNA fragmentation pattern. Our findings suggest that cfDNA fragmentation profiles were associated with physiological parameters related to cardiovascular risk factors, inflammatory response and hepatocyte injury, which may provide insights for further research on the potential role of cfDNA fragmentation in diagnosis and monitor of several disease.

引用

页数：11

共 46 条

[1]

Abramson N, 2000, AM FAM PHYSICIAN, V62, P2053

[2] Trimmomatic: a flexible trimmer for Illumina sequence data [J].

Bolger, Anthony M. ;

Lohse, Marc ;

Usadel, Bjoern .

BIOINFORMATICS, 2014, 30 (15) :2114-2120

[3] Combined C-Reactive Protein and Novel Inflammatory Parameters as a Predictor in Cancer-What Can We Learn from the Hematological Experience? [J].

Bruserud, Oystein ;

Aarstad, Helene Hersvik ;

Tvedt, Tor Henrik Anderson .

CANCERS, 2020, 12 (07) :1-23

[4] Urinary cell-free DNA is a versatile analyte for monitoring infections of the urinary tract [J].

Burnham, Philip ;

Dadhania, Darshana ;

Heyang, Michael ;

Chen, Fanny ;

Westblade, Lars F. ;

Suthanthiran, Manikkam ;

Lee, John Richard ;

De Vlaminck, Iwijn .

NATURE COMMUNICATIONS, 2018, 9

[5] Integrating genomic features for non-invasive early lung cancer detection [J].

Chabon, Jacob J. ;

Hamilton, Emily G. ;

Kurtz, David M. ;

Esfahani, Mohammad S. ;

Moding, Everett J. ;

Stehr, Henning ;

Schroers-Martin, Joseph ;

Nabet, Barzin Y. ;

Chen, Binbin ;

Chaudhuri, Aadel A. ;

Liu, Chih Long ;

Hui, Angela B. ;

Jin, Michael C. ;

Azad, Tej D. ;

Almanza, Diego ;

Jeon, Young-Jun ;

Nesselbush, Monica C. ;

Keh, Lyron Co Ting ;

Bonilla, Rene F. ;

Yoo, Christopher H. ;

Ko, Ryan B. ;

Chen, Emily L. ;

Merriott, David J. ;

Massion, Pierre P. ;

Mansfield, Aaron S. ;

Jen, Jin ;

Ren, Hong Z. ;

Lin, Steven H. ;

Costantino, Christina L. ;

Burr, Risa ;

Tibshirani, Robert ;

Gambhir, Sanjiv S. ;

Berry, Gerald J. ;

Jensen, Kristin C. ;

West, Robert B. ;

Neal, Joel W. ;

Wakelee, Heather A. ;

Loo, Billy W., Jr. ;

Kunder, Christian A. ;

Leung, Ann N. ;

Lui, Natalie S. ;

Berry, Mark F. ;

Shrager, Joseph B. ;

Nair, Viswam S. ;

Haber, Daniel A. ;

Sequist, Lecia V. ;

Alizadeh, Ash A. ;

Diehn, Maximilian .

NATURE, 2020, 580 (7802) :245-+

[6] Plasma DNA aberrations in systemic lupus erythematosus revealed by genomic and methylomic sequencing [J].

Chan, Rebecca W. Y. ;

Jiang, Peiyong ;

Peng, Xianlu ;

Tam, Lai-Shan ;

Liao, Gary J. W. ;

Li, Edmund K. M. ;

Wong, Priscilla C. H. ;

Sun, Hao ;

Chan, K. C. Allen ;

Chiu, Rossa W. K. ;

Lo, Y. M. Dennis .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2014, 111 (49) :E5302-E5311

[7] Cell-free DNA analysis in current cancer clinical trials: a review [J].

Cisneros-Villanueva, M. ;

Hidalgo-Perez, L. ;

Rios-Romero, M. ;

Cedro-Tanda, A. ;

Ruiz-Villavicencio, C. A. ;

Page, K. ;

Hastings, R. ;

Fernandez-Garcia, D. ;

Allsopp, R. ;

Fonseca-Montano, M. A. ;

Jimenez-Morales, S. ;

Padilla-Palma, V ;

Shaw, J. A. ;

Hidalgo-Miranda, A. .

BRITISH JOURNAL OF CANCER, 2022, 126 (03) :391-400

[8] Genome-wide cell-free DNA fragmentation in patients with cancer [J].

Cristiano, Stephen ;

Leal, Alessandro ;

Phallen, Jillian ;

Fiksel, Jacob ;

Adleff, Vilmos ;

Bruhm, Daniel C. ;

Jensen, Sarah Ostrup ;

Medina, Jamie E. ;

Hruban, Carolyn ;

White, James R. ;

Palsgrove, Doreen N. ;

Niknafs, Noushin ;

Anagnostou, Valsamo ;

Forde, Patrick ;

Naidoo, Jarushka ;

Marrone, Kristen ;

Brahmer, Julie ;

Woodward, Brian D. ;

Husain, Hatim ;

van Rooijen, Karlijn L. ;

Orntoft, Mai-Britt Worm ;

Madsen, Anders Husted ;

van de Velde, Cornelis J. H. ;

Verheij, Marcel ;

Cats, Annemieke ;

Punt, Cornelis J. A. ;

Vink, Geraldine R. ;

van Grieken, Nicole C. T. ;

Koopman, Miriam ;

Fijneman, Remond J. A. ;

Johansen, Julia S. ;

Nielsen, Hans Jorgen ;

Meijer, Gerrit A. ;

Andersen, Claus Lindbjerg ;

Scharpf, Robert B. ;

Velculescu, Victor E. .

NATURE, 2019, 570 (7761) :385-+

[9] The Length and Distribution of Plasma Cell-Free DNA Fragments in Stroke Patients [J].

Cui, Xiaofang ;

Du, Shiyi ;

Liu, Houlin ;

Liu, Ju ;

Wu, Qingjian ;

Huo, Qing ;

Qi, Yanwei ;

Qin, Xiao ;

Yang, Yan ;

Li, Weiyang .

BIOMED RESEARCH INTERNATIONAL, 2020, 2020

[10] Circulating Cell-Free DNA Enables Noninvasive Diagnosis of Heart Transplant Rejection [J].

De Vlaminck, Iwijn ;

Valantine, Hannah A. ;

Snyder, Thomas M. ;

Strehl, Calvin ;

Cohen, Garrett ;

Luikart, Helen ;

Neff, Norma F. ;

Okamoto, Jennifer ;

Bernstein, Daniel ;

Weisshaar, Dana ;

Quake, Stephen R. ;

Khush, Kiran K. .

SCIENCE TRANSLATIONAL MEDICINE, 2014, 6 (241)

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