Direct detection and differentiation of Legionella spp. and Legionella pneumophila in clinical specimens by dual-color real-time PCR and melting curve analysis

被引：71

作者：

Reischl, U

Linde, HJ

Lehn, N

Landt, O

Barratt, K

Wellinghausen, N

机构：

[1] Univ Regensburg, Inst Med Mikrobiol & Hyg, D-93053 Regensburg, Germany

[2] TIB Molbiol GmbH, D-10829 Berlin, Germany

[3] Univ Ulm, Inst Med Microbiol & Hyg, D-89081 Ulm, Germany

[4] Hlth Waikato Lab, Hamilton, New Zealand

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2002年 / 40卷 / 10期

关键词：

D O I：

10.1128/JCM.40.10.3814-3817.2002

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A dual-color LightCycler PCR assay targeting the 16S rDNA gene of Legionella spp. was established. By using two pairs of hybridization probes, Legionella spp. and Legionella pneumophila could be detected and differentiated simultaneously. With 26 culture-positive and 42 culture-negative respiratory specimens from patients with atypical pneumonia, 100% sensitivity and specificity was observed for L. pneumophila.

引用

页码：3814 / 3817

页数：4

共 20 条

[11] RAPID DETECTION OF LEGIONELLA SPECIES IN BRONCHOALVEOLAR LAVAGE FLUIDS WITH THE ENVIROAMP LEGIONELLA PCR AMPLIFICATION AND DETECTION KIT
KESSLER, HH
REINTHALER, FF
PSCHAID, A
PIERER, K
KLEINHAPPL, B
EBER, E
MARTH, E
[J]. JOURNAL OF CLINICAL MICROBIOLOGY, 1993, 31 (12) : 3325 - 3328
[12] DIAGNOSIS OF LEGIONELLA-PNEUMOPHILA INFECTION BY POLYMERASE CHAIN-REACTION
KOIDE, M
SAITO, A
[J]. CLINICAL INFECTIOUS DISEASES, 1995, 21 (01) : 199 - 201
[13] Hospital characteristics associated with colonization of water systems by Legionella and risk of nosocomial Legionnaires' disease:: A cohort study of 15 hospitals
Kool, JL
Bergmire-Sweat, D
Butler, JC
Brown, EW
Peabody, DJ
Massi, DS
Carpenter, JC
Pruckler, JM
Benson, RF
Fields, BS
[J]. INFECTION CONTROL AND HOSPITAL EPIDEMIOLOGY, 1999, 20 (12) : 798 - 805
[14] DETECTION OF MIP GENE BY PCR FOR DIAGNOSIS OF LEGIONNAIRES-DISEASE
LINDSAY, DSJ
ABRAHAM, WH
FALLON, RJ
[J]. JOURNAL OF CLINICAL MICROBIOLOGY, 1994, 32 (12) : 3068 - 3069
[15] Use of the polymerase chain reaction to detect Legionella DNA in urine and serum samples from patients with pneumonia
Murdoch, DR
Walford, EJ
Jennings, LC
Light, GJ
Schousboe, MI
Chereshsky, AY
Chambers, ST
Town, GI
[J]. CLINICAL INFECTIOUS DISEASES, 1996, 23 (03) : 475 - 480
[16] NEUMEISTER B, 1996, CLIN LAB, V42, P715
[17] Development of conventional and real-time PCR assays for detection of Legionella DNA in respiratory specimens
Rantakokko-Jalava, K
Jalava, J
[J]. JOURNAL OF CLINICAL MICROBIOLOGY, 2001, 39 (08) : 2904 - 2910
[18] Sequence-based classification scheme for the genus Legionella targeting the mip gene
Ratcliff, RM
Lanser, JA
Manning, PA
Heuzenroeder, MW
[J]. JOURNAL OF CLINICAL MICROBIOLOGY, 1998, 36 (06) : 1560 - 1567
[19] Detection of legionellae in hospital water samples by quantitative real-time LightCycler PCR
Wellinghausen, N
Frost, C
Marre, R
[J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2001, 67 (09) : 3985 - 3993
[20] YU VL, 2000, PRINCIPLES PRACTICE, P2424

← 1 2 →