Direct detection and differentiation of Legionella spp. and Legionella pneumophila in clinical specimens by dual-color real-time PCR and melting curve analysis

被引：71
作者：
Reischl, U
Linde, HJ
Lehn, N
Landt, O
Barratt, K
Wellinghausen, N
机构：
[1] Univ Regensburg, Inst Med Mikrobiol & Hyg, D-93053 Regensburg, Germany
[2] TIB Molbiol GmbH, D-10829 Berlin, Germany
[3] Univ Ulm, Inst Med Microbiol & Hyg, D-89081 Ulm, Germany
[4] Hlth Waikato Lab, Hamilton, New Zealand
来源：
JOURNAL OF CLINICAL MICROBIOLOGY | 2002年 / 40卷 / 10期
关键词：
D O I：
10.1128/JCM.40.10.3814-3817.2002
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
A dual-color LightCycler PCR assay targeting the 16S rDNA gene of Legionella spp. was established. By using two pairs of hybridization probes, Legionella spp. and Legionella pneumophila could be detected and differentiated simultaneously. With 26 culture-positive and 42 culture-negative respiratory specimens from patients with atypical pneumonia, 100% sensitivity and specificity was observed for L. pneumophila.
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页码：3814 / 3817
页数：4
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