Direct detection and differentiation of Legionella spp. and Legionella pneumophila in clinical specimens by dual-color real-time PCR and melting curve analysis

被引：71

作者：

Reischl, U

Linde, HJ

Lehn, N

Landt, O

Barratt, K

Wellinghausen, N

机构：

[1] Univ Regensburg, Inst Med Mikrobiol & Hyg, D-93053 Regensburg, Germany

[2] TIB Molbiol GmbH, D-10829 Berlin, Germany

[3] Univ Ulm, Inst Med Microbiol & Hyg, D-89081 Ulm, Germany

[4] Hlth Waikato Lab, Hamilton, New Zealand

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2002年 / 40卷 / 10期

关键词：

D O I：

10.1128/JCM.40.10.3814-3817.2002

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A dual-color LightCycler PCR assay targeting the 16S rDNA gene of Legionella spp. was established. By using two pairs of hybridization probes, Legionella spp. and Legionella pneumophila could be detected and differentiated simultaneously. With 26 culture-positive and 42 culture-negative respiratory specimens from patients with atypical pneumonia, 100% sensitivity and specificity was observed for L. pneumophila.

引用

收藏

页码：3814 / 3817

页数：4

相关论文

共 20 条

[1] Detection of Legionella pneumophila using a real-time PCR hybridization assay [J].

Ballard, AL ;

Fry, NK ;

Chan, L ;

Surman, SB ;

Lee, JV ;

Harrison, TG ;

Towner, KJ .

JOURNAL OF CLINICAL MICROBIOLOGY, 2000, 38 (11) :4215-4218

[2]

Benson R F, 1998, Semin Respir Infect, V13, P90

[3]

Breiman R F, 1998, Semin Respir Infect, V13, P84

[4] Detection of Legionella species in respiratory specimens using PCR with sequencing confirmation [J].

Cloud, JL ;

Carroll, KC ;

Pixton, P ;

Erali, M ;

Hillyard, DR .

JOURNAL OF CLINICAL MICROBIOLOGY, 2000, 38 (05) :1709-1712

[5]

Domínguez JA, 1998, J CLIN MICROBIOL, V36, P2718

[6]

HARRISON TG, 1998, LANCET, V352, P795

[7] Direct detection of Legionella species from bronchoalveolar lavage and open lung biopsy specimens:: Comparison of LightCycler PCR, in situ hybridization, direct fluorescence antigen detection, and culture [J].

Hayden, RT ;

Uhl, JR ;

Qian, X ;

Hopkins, MK ;

Aubry, MC ;

Limper, AH ;

LLoyd, RV ;

Cockerill, FR .

JOURNAL OF CLINICAL MICROBIOLOGY, 2001, 39 (07) :2618-2626

[8] Delay in appropriate therapy of Legionella pneumonia associated with increased mortality [J].

Heath, CH ;

Grove, DI ;

Looke, DFM .

EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES, 1996, 15 (04) :286-290

[9] ENZYME-LINKED IMMUNOASSAY FOR DETECTION OF PCR-AMPLIFIED DNA OF LEGIONELLAE IN BRONCHOALVEOLAR FLUID [J].

JONAS, D ;

ROSENBAUM, A ;

WEYRICH, S ;

BHAKDI, S .

JOURNAL OF CLINICAL MICROBIOLOGY, 1995, 33 (05) :1247-1252

[10] Rapid diagnosis of Legionella pneumophila serogroup 1 infection with the Binax enzyme immunoassay urinary antigen test [J].

Kazandjian, D ;

Chiew, R ;

Gilbert, GL .

JOURNAL OF CLINICAL MICROBIOLOGY, 1997, 35 (04) :954-956