Cell-intrinsic differences between stem cells from different regions of the peripheral nervous system regulate the generation of neural diversity

被引:235
作者
Bixby, S
Kruger, GM
Mosher, JT
Joseph, NM
Morrison, SJ
机构
[1] Univ Michigan, Howard Hughes Med Inst, Dept Internal Med, Ann Arbor, MI 48109 USA
[2] Univ Michigan, Dept Cell & Dev Biol, Ann Arbor, MI 48109 USA
关键词
D O I
10.1016/S0896-6273(02)00825-5
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
Stem cells in different regions of the nervous system give rise to different types of mature cells. This diversity is assumed to arise in response to local environmental differences, but the contribution of cell-intrinsic differences between stem cells has been unclear. At embryonic day (E)14, neural crest stem cells (NCSCs) undergo primarily neurogenesis in the gut but gliogenesis in nerves. Yet gliogenic and neurogenic factors are expressed in both locations. NCSCs isolated by flow-cytometry from E14 sciatic nerve and gut exhibited heritable, cell-intrinsic differences in their responsiveness to lineage determination factors. Gut NCSCs were more responsive to neurogenic factors, while sciatic nerve NCSCs were more responsive to gliogenic factors. Upon transplantation of uncultured NCSCs into developing peripheral nerves in vivo, sciatic nerve NCSCs gave rise only to glia, while gut NCSCs gave rise primarily to neurons. Thus, cell fate in the nerve was stem cell determined.
引用
收藏
页码:643 / 656
页数:14
相关论文
共 66 条
[1]   MOLECULAR PROBES FOR THE DEVELOPMENT AND PLASTICITY OF NEURAL CREST DERIVATIVES [J].
ANDERSON, DJ ;
AXEL, R .
CELL, 1985, 42 (02) :649-662
[2]   Stem cells and pattern formation in the nervous system: The possible versus the actual [J].
Anderson, DJ .
NEURON, 2001, 30 (01) :19-35
[3]   SUBCELLULAR-LOCALIZATION OF ALPHA-TUBULIN AND OPSIN MESSENGER-RNA IN THE GOLDFISH RETINA USING DIGOXIGENIN-LABELED CRNA PROBES DETECTED BY ALKALINE-PHOSPHATASE AND HRP HISTOCHEMISTRY [J].
BARTHEL, LK ;
RAYMOND, PA .
JOURNAL OF NEUROSCIENCE METHODS, 1993, 50 (02) :145-152
[4]   HEDGEHOG AND BMP GENES ARE COEXPRESSED AT MANY DIVERSE SITES OF CELL-CELL INTERACTION IN THE MOUSE EMBRYO [J].
BITGOOD, MJ ;
MCMAHON, AP .
DEVELOPMENTAL BIOLOGY, 1995, 172 (01) :126-138
[5]   In vitro-generated neural precursors participate in mammalian brain development [J].
Brüstle, O ;
Spiro, AC ;
Karram, K ;
Choudhary, K ;
Okabe, S ;
McKay, RDG .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1997, 94 (26) :14809-14814
[6]   Regional incorporation and site-specific differentiation of striatal precursors transplanted to the embryonic forebrain ventricle [J].
Campbell, K ;
Olsson, M ;
Bjorklund, A .
NEURON, 1995, 15 (06) :1259-1273
[7]  
Couly G, 2002, DEVELOPMENT, V129, P1061
[8]   NEU DIFFERENTIATION FACTOR IS A NEURON-GLIA SIGNAL AND REGULATES SURVIVAL, PROLIFERATION, AND MATURATION OF RAT SCHWANN-CELL PRECURSORS [J].
DONG, Z ;
BRENNAN, A ;
LIU, N ;
YARDEN, Y ;
LEFKOWITZ, G ;
MIRSKY, R ;
JESSEN, KR .
NEURON, 1995, 15 (03) :585-596
[9]   Site-specific migration and neuronal differentiation of human neural progenitor cells after transplantation in the adult rat brain [J].
Fricker, RA ;
Carpenter, MK ;
Winkler, C ;
Greco, C ;
Gates, MA ;
Björklund, W .
JOURNAL OF NEUROSCIENCE, 1999, 19 (14) :5990-6005
[10]   Mammalian neural stem cells [J].
Gage, FH .
SCIENCE, 2000, 287 (5457) :1433-1438