Fluorescent-Protein-Based Biosensors: Modulation of Energy Transfer as a Design Principle

被引:75
作者
Campbell, Robert E. [1 ]
机构
[1] Univ Alberta, Dept Chem, Edmonton, AB T6G 2G2, Canada
基金
加拿大自然科学与工程研究理事会;
关键词
CORRELATION SPECTROSCOPY; FRET; CELLS; REPORTERS; SENSOR; CA2+; NANOSENSORS; INDICATORS; OPTIMIZATION; SENSITIVITY;
D O I
10.1021/ac802613w
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
Genetically-encoded biosensors based on FRET between fluorescent proteins of different hues enable quantitative measurement of intracellular enzyme activities and small molecule concentrations. (To listen to a podcast about this feature, please go to the Analytical Chemistry website at pubs.acs.org/journal/ancham.)
引用
收藏
页码:5972 / 5979
页数:8
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