Bacillus aryabhattai SRB02 tolerates oxidative and nitrosative stress and promotes the growth of soybean by modulating the production of phytohormones

被引:222
作者
Park, Yeon-Gyeong [1 ]
Mun, Bong-Gyu [1 ]
Kang, Sang-Mo [1 ]
Hussain, Adil [1 ,2 ]
Shahzad, Raheem [1 ]
Seo, Chang-Woo [1 ]
Kim, Ah-Yeong [1 ]
Lee, Sang-Uk [1 ]
Oh, Kyeong Yeol [3 ]
Lee, Dong Yeol [3 ]
Lee, In-Jung [1 ]
Yun, Byung-Wook [1 ]
机构
[1] Kyungpook Natl Univ, Sch Appl Biosci, Daegu, South Korea
[2] Abdul Wali Khan Univ, Dept Agr, Mardan, Khyber Pakhtunk, Pakistan
[3] Gyeongnam Oriental Med Herb Inst, Sancheong, South Korea
基金
新加坡国家研究基金会;
关键词
PHYTOCHROME-MEDIATED ATTACHMENT; INDUCED SYSTEMIC RESISTANCE; ABSCISIC-ACID ANTAGONISM; PLANT-GROWTH; HEAT-STRESS; CYTOKININ PRODUCTION; PSEUDOMONAS-AERUGINOSA; INDOLEACETIC-ACID; DROUGHT STRESS; SALICYLIC-ACID;
D O I
10.1371/journal.pone.0173203
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Plant growth promoting rhizobacteria (PGPR) are diverse, naturally occurring bacteria that establish a close association with plant roots and promote the growth and immunity of plants. Established mechanisms involved in PGPR-mediated plant growth promotion include regulation of phytohormones, improved nutrient availability, and antagonistic effects on plant pathogens. In this study, we isolated a bacterium from the rhizospheric soil of a soybean field in Chungcheong buk-do, South Korea. Using 16S rRNA sequencing, the bacterium was identified as Bacillus aryabhattai strain SRB02. Here we show that this strain significantly promotes the growth of soybean. Gas chromatography-mass spectrometry analysis showed that SRB02 produced significant amounts of abscisic acid, indole acetic acid, cytokinin and different gibberellic acids in culture. SRB02-treated soybean plants showed significantly better heat stress tolerance than did untreated plants. These plants also produced consistent levels of ABA under heat stress and exhibited ABA-mediated stomatal closure. High levels of IAA, JA, GA12, GA4, and GA7, were recorded in SRB02-treated plants. These plants produced longer roots and shoots than those of control plants. B. aryabhattai SRB02 was found to be highly tolerant to oxidative stress induced by H2O2 and MV potentiated by high catalase (CAT) and superoxide dismutase (SOD) activities. SRB02 also tolerated high nitrosative stress induced by the nitric oxide donors GSNO and CysNO. Because of these attributes, B. aryabhattai SRB02 may prove to be a valuable resource for incorporation in biofertilizers and other soil amendments that seek to improve crop productivity.
引用
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页数:28
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