Molecular evaluation of drug resistance in clinical isolates of Salmonella enterica serovar Typhi from Pakistan

被引：18

作者：

Afzal, Amna ^{[1
]}

Sarwar, Yasra ^{[1
]}

Ali, Aamir ^{[1
]}

Maqbool, Abbas ^{[2
]}

Salman, Muhammad ^{[1
]}

Habeeb, Muhammad Asif ^{[1
]}

Haque, Abdul ^{[1
]}

机构：

[1] Natl Inst Biotechnol & Genet Engn, Hlth Biotechnol Div, Faisalabad, Pakistan

[2] Univ York, Dept Biol, York YO10 5DD, N Yorkshire, England

来源：

JOURNAL OF INFECTION IN DEVELOPING COUNTRIES | 2013年 / 7卷 / 12期

关键词：

Salmonella Typhi; multidrug resistance; fluoroquinolone resistance; MULTIPLE ANTIBIOTIC-RESISTANCE; MULTIDRUG-RESISTANCE; ANTIMICROBIAL RESISTANCE; QUINOLONE RESISTANCE; BETA-LACTAMASE; DECREASED SUSCEPTIBILITY; SPECTRUM CEPHALOSPORINS; REDUCED SUSCEPTIBILITY; SEROTYPE TYPHIMURIUM; DETERMINING REGION;

D O I：

10.3855/jidc.3154

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

Introduction: This study aimed to determine the drug susceptibility patterns and genetic elements related to drug resistance in isolates of Salmonella enterica serovar Typhi (S. Typhi) from the Faisalabad region of Pakistan. Methodology: The drug resistance status of 80 isolates were evaluated by determining antimicrobial susceptibility, MICs, drug resistance genes involved, and the presence of integrons. Nalidixic acid resistance and reduced susceptibility to ciprofloxacin were also investigated by mutation screening of the gyrA, gyrB, parC, and parE genes. Results: Forty-seven (58.7%) isolates were multidrug resistant (MDR). Among the different resistance (R) types, the most commonly observed (13/80) was AmChStrTeSxtSmzTmp, which is the most frequent type observed in India and Pakistan. The most common drug resistant genes were bla(TEM-1), cat, strA-strB, tetB, sul1, sul2, and dfrA7. Among the detected genes, only dfrA7 was found to be associated in the form of a single gene cassette within the class 1 integrons. Conclusions: MIC determination of currently used drugs revealed fourth-generation gatifloxacin as an effective drug against multidrug-resistant S. Typhi, but its clinical use is controversial. The Ser83 -> Phe substitution in gyrA was the predominant alteration in nalidixic acid-resistant isolates, exhibiting reduced susceptibility and increased MICs against ciprofloxacin. No mutations in gyrB, parC, or parE were detected in any isolate.

引用

页码：929 / 940

页数：12

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