Glucose oxidation catalyzed by FAD-dependent glucose dehydrogenase within Os complex-tethered redox polymer hydrogel

被引：44

作者：

Murata, Kazuki ^{[1
]}

Akatsuka, Wataru ^{[1
]}

Sadakane, Takuya ^{[1
]}

Matsunaga, Aya ^{[1
]}

Tsujimura, Seiya ^{[1
]}

机构：

[1] Univ Tsukuba, Fac Pure & Appl Sci, Tsukuba, Ibaraki 3058573, Japan

来源：

ELECTROCHIMICA ACTA | 2014年 / 136卷

关键词：

Glucose dehydrogenase; Wiring; Os complex; Blood glucose sensor; Biofuel cell; BIOFUEL CELLS; ENZYME ELECTRODES; CARBON NANOTUBES; MICROSCALE; FILMS;

D O I：

10.1016/j.electacta.2014.05.088

中图分类号：

O646 [电化学、电解、磁化学];

学科分类号：

081704 ;

摘要：

FAD-dependent glucose dehydrogenase (FAD-GDH) from Aspergillus terreus was co-immobilized on a glassy carbon (GC) electrode surface with a poly(1-vinylimidazole)-tethered Os(2,2'-bipyridine)(2)Cl complex as a redox mediator. The steady-state catalytic current for glucose oxidation was 2.6 mA cm(-2) at pH 7 and 25 degrees C. This value increased 1.6-fold after oxidative deglycosylation of the enzyme, which is the highest value so far reported for a GC-electrode-based glucose anode. The deglycosylation process did not decrease the stability of the FAD-GDH dissolved in the buffer solution and immobilized within the hydrogel. A 10% decrease in the catalytic current was observed after 24h continuous operation. (C) 2014 Elsevier Ltd. All rights reserved.

引用

页码：537 / 541

页数：5

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