Rapid dry-reagent immunomagnetic biosensing platform based on volumetric detection of nanoparticles on 3D structures

被引：73
作者：
Orlov, Alexey V. [1 ,2 ]
Bragina, Vera A. [1 ]
Nikitin, Maxim P. [1 ,2 ]
Nikitin, Petr I. [1 ]
机构：
[1] Russian Acad Sci, Prokhorov Gen Phys Inst, 38 Vavilov St, Moscow 119991, Russia
[2] State Univ, Moscow Inst Phys & Technol, 9 Inst Per, Dolgoprudnyi 141700, Moscow Region, Russia
来源：
BIOSENSORS & BIOELECTRONICS | 2016年 / 79卷
基金：
俄罗斯基础研究基金会;
关键词：
Magnetic particle quantification; Biosensor; Lateral flow; In-vitro diagnostics; Prostate-specific antigen; Non-linear magnetization; MAGNETIC MICROSPHERES; CELL-SEPARATION; DRUG-DELIVERY; IMMUNOASSAY; ASSAY; MATRICES;
D O I：
10.1016/j.bios.2015.12.049
中图分类号：
Q6 [生物物理学];
学科分类号：
071011 ;
摘要：
A dry-reagent immunomagnetic (DRIM) biosensing platform is developed for rapid high-precision quantitative analyses for in vitro diagnostics. The platform combines the advantages of immunochromatography with highly sensitive quantification of 200-nm magnetic nanoparticles (MP) from the entire volume of lateral flow membranes. The MP are registered by their non-linear magnetization at combinatorial frequencies by a portable reader that offers the detection limit of 60 zeptomoles or 0.4 ng of magnetic nanolabels and extremely wide linear dynamic range of 7 orders. The efficient combination of small MP with large reaction surface of the 3D membranes has permitted the detection in human serum of as low as 25 pg/ml total prostate specific antigen (PSA) during 30-min assay. The featured 3-fold signal increase per every order of concentration within 3.5 orders of magnitude allows precise analysis of antigen concentrations in a wide range. The system also provides the first tool for quantitative MP mapping along the lateral flow strip for easy development and optimization of assays. The DRIM detection can be used for simple, rapid and sensitive quantification of protein biomarkers for in vitro diagnostics both in laboratory and near-patient conditions, for food analysis, environmental monitoring, security, and safety applications. (C) 2015 Elsevier B.V. All rights reserved.
引用
收藏
页码：423 / 429
页数：7
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