Study of SiRNA-loaded PS-mPEG/CaP nanospheres on lung cancer

被引：3

作者：

Wang, Qi ^{[1
,2
]}

Qin, Liubin ^{[1
]}

Sun, Ying ^{[1
]}

Shen, Ming ^{[1
]}

Duan, Yourong ^{[1
]}

机构：

[1] Shanghai Jiao Tong Univ, State Key Lab Oncogenes & Related Genes, Shanghai Canc Inst, Renji Hosp,Sch Med, Shanghai 200032, Peoples R China

[2] Sichuan Univ, Key Lab Drug Targeting & Novel Drug Delivery Syst, Minist Educ, West China Sch Pharm, Chengdu 610041, Sichuan, Peoples R China

来源：

JOURNAL OF NANOPARTICLE RESEARCH | 2014年 / 16卷 / 05期

基金：

美国国家科学基金会;

关键词：

Ultrasonic-absorption method; Bcl-2-SiRNA; Gene silencing; Targeting; Lung cancer; Nanomedicine; GOLD NANOPARTICLES; CALCIUM-PHOSPHATE; APOPTOSIS; CELLS; THERAPEUTICS; CYTOTOXICITY; REVOLUTION; MECHANISM; HEPATOMA; DELIVERY;

D O I：

10.1007/s11051-014-2421-3

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

An ultrasound-adsorption method was used to prepare Bcl-2-SiRNA-loaded PS-mPEG/CaP nanospheres. The size and zeta potential were 18.41 +/- 4.31 nm (n = 5) and -23.5 +/- 0.6 mV, respectively. The entrapment efficiency of SiRNA was 92.86 %. MTT assay results confirmed that the blank nanospheres demonstrated a negligible cytotoxicity response in H1299 cells. Flow cytometer analysis results demonstrated that PS-mPEG/CaP NSs could carry SiRNA into the cells effectively. RT-PCR experiments and apoptosis assay results approved that, compared with free SiRNA, SiRNA-loaded PS-mPEG/CaP NSs could silence Bcl-2 gene and induce cell apoptosis effectively. In vivo distribution results confirmed PS-mPEG/CaP NSs could carry SiRNA enter the tumor tissue effectively. Taken together, these results suggest that the Bcl-2-SiRNA-loaded PS-mPEG/CaP nanospheres have great potential to be used to cure lung cancer.

引用

页数：9

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