Highly sensitive covalently functionalised integrated silicon nanowire biosensor devices for detection of cancer risk biomarker

被引：69

作者：

Azmi, M. A. Mohd ^{[1
,3
]}

Tehrani, Z. ^{[1
]}

Lewis, R. P. ^{[1
]}

Walker, K. -A. D. ^{[1
]}

Jones, D. R. ^{[1
]}

Daniels, D. R. ^{[1
]}

Doak, S. H. ^{[2
]}

Guy, O. J. ^{[1
]}

机构：

[1] Swansea Univ, Coll Engn, Swansea, W Glam, Wales

[2] Swansea Univ, Coll Med, Swansea, W Glam, Wales

[3] Univ Kuala Lumpur, British Malaysian Inst, Gombak, Selangor, Malaysia

来源：

BIOSENSORS & BIOELECTRONICS | 2014年 / 52卷

关键词：

Silicon nanowire; Biosensor; Surface functionalisation; Electron beam lithography; 8-hydroxydeoxyguanosine (8-OHdG); Electronic readout; ELECTRICAL DETECTION; NANOSENSORS; FABRICATION; SURFACES; ARRAYS;

D O I：

10.1016/j.bios.2013.08.030

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

In this article we present ultra-sensitive, silicon nanowire (SiNW)-based biosensor devices for the detection of disease biomarkers. An electrochemically induced functionalisation method has been employed to graft antibodies targeted against the prostate cancer risk biomarker 8-hydroxydeoxyguanosine (8-OHdG) to SiNW surfaces. The antibody-functionalised SiNW sensor has been used to detect binding of the 8-OHdG biomarker to the SiNW surface within seconds of exposure. Detection of 8-OHdG concentrations as low as 1 ng/ml (3.5 nM) has been demonstrated. The active device has been bonded to a disposable printed circuit which can be inserted into an electronic readout system as part of an integrated Point of Care (POC) diagnostic. The speed, sensitivity and ease of detection of biomarkers using SiNW sensors render them ideal for eventual POC diagnostics. Crown Copyright (C) 2013 Published by Elsevier B.V. All rights reserved.

引用

页码：216 / 224

页数：9

共 31 条

[1] Double-Gate Nanowire Field Effect Transistor for a Biosensor [J].

Ahn, Jae-Hyuk ;

Choi, Sung-Jin ;

Han, Jin-Woo ;

Park, Tae Jung ;

Lee, Sang Yup ;

Choi, Yang-Kyu .

NANO LETTERS, 2010, 10 (08) :2934-2938

[2] Covalent modification of carbon surfaces by aryl radicals generated from the electrochemical reduction of diazonium salts [J].

Allongue, P ;

Delamar, M ;

Desbat, B ;

Fagebaume, O ;

Hitmi, R ;

Pinson, J ;

Saveant, JM .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1997, 119 (01) :201-207

[3]

[Anonymous], 2005, NANOSCALE SCI TECHNO

[4] Reliability of TCT and HH/HT test performed in chips and flex substrates assembled by thermosonic flip-chip bonding process [J].

Aoh, Jong-Ning ;

Chuang, Cheng-Li ;

Kang, Min-Yi .

MICROELECTRONICS RELIABILITY, 2013, 53 (03) :463-472

[5] Nanowire nanosensors for highly sensitive and selective detection of biological and chemical species [J].

Cui, Y ;

Wei, QQ ;

Park, HK ;

Lieber, CM .

SCIENCE, 2001, 293 (5533) :1289-1292

[6] High performance silicon nanowire field effect transistors [J].

Cui, Y ;

Zhong, ZH ;

Wang, DL ;

Wang, WU ;

Lieber, CM .

NANO LETTERS, 2003, 3 (02) :149-152

[7] Doping and electrical transport in silicon nanowires [J].

Cui, Y ;

Duan, XF ;

Hu, JT ;

Lieber, CM .

JOURNAL OF PHYSICAL CHEMISTRY B, 2000, 104 (22) :5213-5216

[8] Silicon nanoribbons for electrical detection of biomolecules [J].

Elfstroem, Niklas ;

Karlstroem, Amelie Eriksson ;

Linnros, Jan .

NANO LETTERS, 2008, 8 (03) :945-949

[9] Biomarker evidence of DNA oxidation in lung cancer patients: Association of urinary 8-hydroxy-2'-deoxyguanosine excretion with radiotherapy, chemotherapy, and response to treatment [J].

Erhola, M ;

Toyokuni, S ;

Okada, K ;

Tanaka, T ;

Hiai, H ;

Ochi, H ;

Uchida, K ;

Osawa, T ;

Nieminen, MM ;

Alho, H ;

KellokumpuLehtinen, P .

FEBS LETTERS, 1997, 409 (02) :287-291

[10] Silicon-Nanowire-Based CMOS-Compatible Field-Effect Transistor Nanosensors for Ultrasensitive Electrical Detection of Nucleic Acids [J].

Gao, Anran ;

Lu, Na ;

Dai, Pengfei ;

Li, Tie ;

Pei, Hao ;

Gao, Xiuli ;

Gong, Yibin ;

Wang, Yuelin ;

Fan, Chunhai .

NANO LETTERS, 2011, 11 (09) :3974-3978

← 1 2 3 4 →