Effects of lysophosphatidylcholine on β-amyloid-induced neuronal apoptosis

被引:23
作者
Qin, Zhen-xia [1 ]
Zhu, Hui-yan [1 ]
Hu, Ying-he [1 ]
机构
[1] E China Normal Univ, Shanghai Inst Brain Funct Gen, MOE&STCSM, Key Lab Brain Funct Gen, Shanghai 200062, Peoples R China
关键词
amyloid beta (1-42) peptide; lysophosphatidylcholine; neuronal apoptosis; ALZHEIMERS-DISEASE; PC12; CELLS; HIPPOCAMPAL-NEURONS; INHIBITS APOPTOSIS; SH-SY5Y CELLS; A-BETA; PEPTIDE; DEATH; BRAIN; NEURODEGENERATION;
D O I
10.1038/aps.2009.25
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
Aim: We have investigated the effects of lysophosphatidylcholine (LPC), a product of lipid peroxidation, on A beta(1-42)-induced SH-SY5Y cell apoptosis. Methods: The viability of cultured SH-SY5Y cells was measured using a CCK-8 kit. Apoptosis was determined by Chip-based flow cytometric assay. The mRNA transcription of Bcl-2, Bax, and caspase-3 were detected by using reverse transcription and real-time quantitative PCR and the protein levels of Bax and caspase-3 were analyzed by Western blotting. The cytosolic calcium concentration of SH-SY5Y cells was tested by calcium influx assay. G2A expression in SH-SY5Y cells was silenced by small interfering RNA. Results: Long-term exposure of SH-SY5Y cells to LPC augmented the neurotoxicity of A beta(1-42). Furthermore, after LPC treatment, the Bax/Bcl-x(L) ratio and the expression levels, as well as the activity of caspase-3 were, elevated, whereas the expression level of TRAF1 was reduced. Because LPC was reported to be a specific ligand for the orphan G-protein coupled receptor, G2A, we investigated LPC-mediated changes in calcium levels in SH-SY5Y cells. Our results demonstrated that LPC can enhance the A beta(1-42)-induced elevation of intracellular calcium. Interestingly, A beta(1-42)-significantly increased the expression of G2A in SH-SY5Y cells, whereas knockdown of G2A using siRNA reduced the effects of LPC on A beta(1-42)-induced neurotoxicity. Conclusion: The effects of LPC on A beta(1-42)-induced apoptosis may occur through the signal pathways of the orphan G-protein coupled receptor.
引用
收藏
页码:388 / 395
页数:8
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