Long-read sequencing in fungal identification

被引:7
作者
Hoang, Minh Thuy Vi [1 ,2 ]
Irinyi, Laszlo [1 ,2 ,3 ]
Meyer, Wieland [1 ,2 ,3 ,4 ,5 ]
机构
[1] Univ Sydney, Fac Med & Hlth, Sydney Med Sch, Westmead Clin Sch,Ctr Infect Dis & Microbiol,Mol, Sydney, NSW 2006, Australia
[2] Westmead Inst Med Res, Ctr Infect Dis & Microbiol, Mol Mycol Res Lab, Westmead, NSW 2145, Australia
[3] Univ Sydney, Sydney Inst Infect Dis, Sydney, NSW 2006, Australia
[4] Westmead Hosp, Res & Educ Network, Westmead, NSW 2145, Australia
[5] Curtin Univ, Curtin Med Sch, Perth, WA 6102, Australia
基金
英国医学研究理事会;
关键词
diagnostics; DNA barcoding; fungal identification; long-read sequencing; metabarcoding; metagenomics; mycoses; next generation sequencing;
D O I
10.1071/MA22006
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Long-read sequencing is currently supported by sequencing platforms from Pacific Biosciences and Oxford Nanopore Technologies, both of which generate ultra-long reads. Metabarcoding and metagenomics are the two approaches used when implementing sequencing. Metabarcoding involves the amplification and sequencing of selected nucleic acid regions, while in a metagenomic approach extracted nucleic acids are sequenced directly without prior amplification. Both approaches have associated advantages and disadvantages, which, in combination with long-read sequencing, provide a promising new approach for fungal identification and diagnosis of mycoses, on which we will reflect in this short review.
引用
收藏
页码:14 / 18
页数:5
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