Preparative separation of flavonoids in Adinandra nitida leaves by high-speed counter-current chromatography and their effects on human epidermal carcinoma cancer cells

被引：56

作者：

Yuan, Erdong ^{[2
]}

Liu, Benguo ^{[1
]}

Ning, Zhengxiang ^{[2
]}

Chen, Chungang ^{[1
]}

机构：

[1] Henan Inst Sci & Technol, Sch Food Sci, Xinxiang 453003, Henan, Peoples R China

[2] S China Univ Technol, Coll Light Ind & Food Sci, Guangzhou 510640, Guangdong, Peoples R China

来源：

FOOD CHEMISTRY | 2009年 / 115卷 / 03期

关键词：

Flavonoid; Camellianin A; High-speed counter-current chromatography; Adinandra nitida; Cytotoxicity; PROLIFERATION; ANTIOXIDANT;

D O I：

10.1016/j.foodchem.2009.01.009

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

Preparative separation of camellianins A and B in Adinandra nitida leaves, which is a flavonoid-rich plant source with great practical prospects, was conducted by high-speed counter-current chromatography (HSCCC) with a solvent system composed of ethyl acetate-ethanol-water (5:1:5, v/v). Apigenin (their aglycone) was prepared by hydrolysing a water extract and recrystallising. The purity and identity of these flavonoids was confirmed by HPLC-ESI/MS, and H-1 and C-13 NMR. These flavones were used in cultures of the A431 cell line. Their inhibitory effect was evaluated by using the MTT assay. The results showed that three flavones could significantly inhibit the growth of human epidermal carcinoma cancer cells in a concentration-dependent manner. After 48 h of treatment, IC50 values of camellianin A, camellianin B and apigenin against A431 turnout cells were 9.09 mu M, 12.5 mu M, 21.0 mu M, respectively. (C) 2009 Elsevier Ltd. All rights reserved.

引用

页码：1158 / 1163

页数：6

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