Physicochemical properties of starches and expression and activity of starch biosynthesis-related genes in sweet potatoes

被引：75
作者：
Lai, Yung C. [1 ]
Wang, Shu Y. [3 ]
Gao, Huan Y. [3 ]
Nguyen, Khiem M. [2 ]
Nguyen, Chinh H. [2 ]
Shih, Ming C. [4 ]
Lin, Kuan H. [2 ,3 ]
机构：
[1] Chiayi Agr Expt Stn, Dept Agron, Chiayi 600, Taiwan
[2] Ton Duc Thang Univ, Fac Sci Appl, Ho Chi Minh 700000, Vietnam
[3] Chinese Culture Univ, Grad Inst Biotechnol, Taipei 111, Taiwan
[4] Chinese Culture Univ, Dept Nutr & Hlth Sci, Taipei 111, Taiwan
来源：
FOOD CHEMISTRY | 2016年 / 199卷
关键词：
Sweet potato starch; Amylose content; Gelatinization; Pasting; Starch-biosynthesis enzyme; BRANCHING ENZYME-I; RICE STARCHES; FUNCTIONAL-PROPERTIES; AMYLOSE; AMYLOPECTIN; IMPACT; WHEAT; INHIBITION; METABOLISM; ISOFORMS;
D O I：
10.1016/j.foodchem.2015.12.053
中图分类号：
O69 [应用化学];
学科分类号：
081704 ;
摘要：
The functional properties of starches from six sweet potato varieties containing various starch components and structures were studied in an attempt to identify starch sources for industrial uses. Tainan 18 (TNN18) with high-amylose (AM) starch exhibited high setback and breakdown viscosities, high water solubility at 85 degrees C but low swelling volume at 65 degrees C, and high hardness and adhesiveness; in contrast, the low-AM starch of Tainung 31 (TNG31) had opposite characteristics. Seven genes related to starch biosynthesis were tested, and GBSS, SS, SBEII, ISA, and AGPase were highly expressed in TNN18 and TNG31; however, transcript levels in DBE and SBE were extremely low. GBSS and SS activity reflected the abundance of GBSS and SS mRNA in TNG31 and TNN18, and expression of AGPase, GBSS, SS, and SBE in TNN18 substantially increased content of AM. The expression and activity of DBE had a significant effect on TNG31 with increased AP content. (C) 2015 Elsevier Ltd. All rights reserved.
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收藏
页码：556 / 564
页数：9
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