Lateral resolution enhancement with standing evanescent waves

被引：106

作者：

Cragg, GE ^{[1
]}

So, PTC ^{[1
]}

机构：

[1] MIT, Dept Mech Engn, Cambridge, MA 02139 USA

来源：

OPTICS LETTERS | 2000年 / 25卷 / 01期

关键词：

D O I：

10.1364/OL.25.000046

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

A high-resolution fluorescence microscopy technique has been developed that achieves a lateral resolution of better than one sixth of the emission wavelength (FWHM). By use of a total-internal-reflection geometry, standing evanescent waves are generated that spatially modulate the excitation of the sample. An enhanced two-dimensional image is formed from a weighted sum of images taken at different phases and directions of the standing wave. The performance of such a system is examined through theoretical calculations of both the point-spread function and the optical transfer function. (C) 2000 Optical Society of America.

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页码：46 / 48

页数：3

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