Selection and Validation of Candidate Housekeeping Genes for Studies of Human Keratinocytes - Review and Recommendations

被引：38

作者：

Baer, Michael ^{[1
]}

Baer, Dorit ^{[2
]}

Lehmann, Bodo ^{[1
]}

机构：

[1] Carl Gustav Carus Med Sch, Dept Dermatol, D-01307 Dresden, Germany

[2] Tech Univ Dresden, Carl Gustav Carus Med Sch, Dept Psychiat, D-8027 Dresden, Germany

来源：

JOURNAL OF INVESTIGATIVE DERMATOLOGY | 2009年 / 129卷 / 03期

关键词：

GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE; BETA-ACTIN; RNA LEVELS; EXPRESSION; NORMALIZATION; PCR;

D O I：

10.1038/jid.2008.428

中图分类号：

R75 [皮肤病学与性病学];

学科分类号：

100206 ;

摘要：

Gene expression analysis using real-time PCR has become an integral part of biomedical research. Appropriate data normalization based on stably expressed housekeeping genes is crucial for reliable results. Thus, candidate housekeeping genes require careful evaluation with regard to the individual experimental system before being selected for studies of human keratinocytes. Future research may be based on published data, as provided by Minner and Poumay in this issue.

引用

页码：535 / 537

页数：4

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