Single Nucleotide Polymorphism in Protamine 1 and Protamine 2 genes in fertile and infertile for men of Al-Najaf City

Around 15% of the couples on the planet confront failure in the primary involvement in pregnancy. These issues in these couples can be explained as infertility. The objective of the study was to determination relationship among polymorphisms Protamin1 (G197T) and Protamine2 (C248T) were studied in three groups (Teratozoospermia n=37, infertile normozoospermia n=34 compare with fertile normozoospermia 17=0 ). Methods: Samples were collected at the Fertility Center Laboratories in Sadr Medical City. Analysis of SNPs was performed for PRM1 and PRM2 by using restriction fragment length polymorphism (PCR-RFLP). Results: The result showed Three types of polymorphism occur in both PRM1 (G197, G197T and G/T197) and PRM2 (C248, C248TandC/T248) genes found in all our patients. In conclusion the results are consistent with some previous studies and indicating that all our tested with SNPs was associated with teratospermia and normospermia as idiopatic male infertility in Iraqi population, which can find a genetically engineered solution in the future.