Surface plasmon resonance biosensors for detection of Alzheimer disease biomarker

被引：63

作者：

Hegnerova, K. ^{[1
]}

Bockova, M. ^{[1
]}

Vaisocherova, H. ^{[1
]}

Kristofikova, Z. ^{[2
]}

Ricny, J. ^{[2
]}

Ripova, D. ^{[2
]}

Homola, J. ^{[1
]}

机构：

[1] Inst Photon & Elect AS CR, Dept Opt Sensors, Prague 18251 8, Czech Republic

[2] Prague Psychiat Ctr, Alzheimer Dis Ctr, Prague 8, Czech Republic

来源：

SENSORS AND ACTUATORS B-CHEMICAL | 2009年 / 139卷 / 01期

关键词：

Alzheimer disease; 17; beta-HSD10; Amyloid beta; SPR sensor; A-BETA; DIAGNOSIS; SENSORS; TAU;

D O I：

10.1016/j.snb.2008.09.006

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

This paper describes direct label-free detection of 17beta-hydroxysteroid dehydrogenase type 10 (17 beta-HSD10) using a surface plasmon resonance (SPR) biosensor. This multifunctional mitochondrial enzyme is involved in pathogenesis of Alzheimer disease (AD) and represents a potential target for AD diagnostics. A multichannel SPR sensor with spectral modulation is functionalized with amyloid beta or polyclonal antibody against 17 beta-HSD10 peptide using a self-assembled monolayer of alkylthiolates and amino Coupling chemistry. Detection of a synthetic peptide of 14 amino acids from the 17 beta-HSD10 molecule (residues 133-146aa) and whole enzyme 17 beta-HSD10 is performed. Detection of 17 beta-HSD10 enzyme in artificial cerebrospinal fluid (ACSF) buffer is also carried out. It is demonstrated that the reported SPR biosensor is capable of detecting 17 beta-HSD10 enzyme at ng/ml levels. (C) 2008 Elsevier B.V. All rights reserved.

引用

页码：69 / 73

页数：5

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