Transformation of trichothecene mycotoxins by microorganisms from fish digesta

被引：81

作者：

Guan, Shu ^{[1
,2
]}

He, Jianwei ^{[1
]}

Young, J. Christopher ^{[1
]}

Zhu, Honghui ^{[1
]}

Li, Xiu-Zhen ^{[1
]}

Ji, Cheng ^{[2
]}

Zhou, Ting ^{[1
]}

机构：

[1] Agr & Agri Food Canada, Guelph Food Res Center, Guelph, ON N1G 5C9, Canada

[2] China Agr Univ, Natl Key Lab Anim Nutr, Beijing 100193, Peoples R China

来源：

AQUACULTURE | 2009年 / 290卷 / 3-4期

基金：

中国国家自然科学基金;

关键词：

Ameriurus nebulosus; Catfish; Microbial culture; Trichothecene; Mycotoxin; Transformation; T-2; TOXIN; MICROBIAL TRANSFORMATION; CTENOPHARYNGODON-IDELLA; BACTERIAL-FLORA; GRASS CARP; DEOXYNIVALENOL; METABOLISM; DETOXIFICATION; DIACETOXYSCIRPENOL; DEGRADATION;

D O I：

10.1016/j.aquaculture.2009.02.037

中图分类号：

S9 [水产、渔业];

学科分类号：

0908 ;

摘要：

Trichothecene mycotoxins are commonly found in cereals worldwide and bring significant threats to the food industry and animal production. The aim of this research was to search for microbes from fish guts capable of transforming trichothecenes into less toxic compounds. Digesta of 62 fishes from nine species were screened for their ability to transform 4-deoxynivalenol (DON). Liquid chromatography-mass spectrometry was used to determine the reduction of DON concentrations and structures of DON-transformation products. The microbial community from one catfish Ameiurus nebulosus, namely microbial culture C133, completely transformed DON to deepoxy DON (dE-DON) at 15 degrees C in full medium after 96 h incubation. Various media and culture conditions were tested to evaluate their effect on DON transformation. Microbial culture C133 maintained high transformation ability over a broad range of temperatures from 4 to 25 degrees C and pH values from 4.5 to 10.4. The transformation of DON to dE-DON was enhanced in a rich medium such as full medium, nutrient broth and corn meal broth. Microbial culture C133 was then tested for its ability to transform other trichothecene mycotoxins; most of the toxins were transformed to deacetyl and/or deepoxy products. This is the first report on trichothecene transformation by microbes from the intestinal tract of fish. Crown Copyright (C) 2009 Published by Elsevier B.V. All rights reserved.

引用

页码：290 / 295

页数：6

共 45 条

[1]

[Anonymous], ALLT 2 AQ WORKSH DUN

[2] Fish model for assessing the in vivo estrogenic potency of the mycotoxin zearalenone and its metabolites [J].

Arukwe, A ;

Grotmol, T ;

Haugen, TB ;

Knudsen, FR ;

Goksoyr, A .

SCIENCE OF THE TOTAL ENVIRONMENT, 1999, 236 (1-3) :153-161

[3] Enzyme producing bacterial flora isolated from fish digestive tracts [J].

Bairagi, A ;

Ghosh, KS ;

Kumar, S ;

Sen, SK ;

Ray, AK .

AQUACULTURE INTERNATIONAL, 2002, 10 (02) :109-121

[4] BIOTRANSFORMATION AND DETOXIFICATION OF T-2 TOXIN BY SOIL AND FRESH-WATER BACTERIA [J].

BEETON, S ;

BULL, AT .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1989, 55 (01) :190-197

[5]

DAVID W, 1994, J VET DIAGN INVEST, V6, P123

[6]

Desjardins A.E., 2006, FUSARIUM MYCOTOXINS

[7]

DIAZ DE, 2005, MYCOTOXIN BLUE BOOK, P145

[8] PROTEIN-SYNTHESIS INHIBITION BY 8-OXO-12,13-EPOXYTRICHOTHECENES [J].

EHRLICH, KC ;

DAIGLE, KW .

BIOCHIMICA ET BIOPHYSICA ACTA, 1987, 923 (02) :206-213

[9] The role of bacteria in the digestion of seaweed by the abalone Haliotis midae [J].

Erasmus, JH ;

Cook, PA ;

Coyne, VE .

AQUACULTURE, 1997, 155 (1-4) :377-386

[10] Comparative cytotoxicity of deoxynivalenol, nivalenol, their acetylated derivatives and de-epoxy metabolites [J].

Eriksen, GS ;

Pettersson, H ;

Lundh, T .

FOOD AND CHEMICAL TOXICOLOGY, 2004, 42 (04) :619-624

← 1 2 3 4 5 →