Dehydration mechanism of optical clearing in tissue

被引：126

作者：

Rylander, Christopher G.

Stumpp, Oliver F.

Milner, Thomas E.

Kemp, Nate J.

Mendenhall, John M.

Diller, Kenneth R.

Welch, A. J.

机构：

[1] Virginia Tech, Dept Mech Engn, Corp Res Ctr, Blacksburg, VA 24061 USA

[2] Virginia Tech, SBES, Corp Res Ctr, Blacksburg, VA 24061 USA

[3] Univ Texas, Dept Biomed Engn, Austin, TX 78712 USA

[4] Univ Texas, Inst Cellular & Mol Biol, Austin, TX 78712 USA

来源：

JOURNAL OF BIOMEDICAL OPTICS | 2006年 / 11卷 / 04期

基金：

美国国家科学基金会;

关键词：

dehydration; optical clearing; density; scattering coefficient; refractive index; cell organelle; collagen fibril; optical coherence tomography; transmission electron microscopy;

D O I：

10.1117/1.2343208

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Previous studies identified various mechanisms of light scattering reduction in tissue induced by chemical agents. Our results suggest that dehydration is an important mechanism of optical clearing in collagenous and cellular tissue. Photographic and optical coherence tomography images indicate that air-immersed skin and tendon specimens become similarly transparent to glycerol-immersed specimens. Transmission electron microscopy images reveal that dehydration causes individual scattering particles such as collagen fibrils and organelles to become more densely packed, but does not significantly alter size. A heuristic particle-interaction model predicts that the scattering particle volume fraction increase can contribute substantially to optical clearing in collagenous and cellular tissue. (c) 2006 Society of Photo-Optical Instrumentation Engineers.

引用

页数：7

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