Construction of a novel stigmatic MALDI imaging mass spectrometer

被引：34

作者：

Hazama, Hisanao ^{[1
,4
]}

Aoki, Jun ^{[2
,4
]}

Nagao, Hirofumi ^{[1
,4
]}

Suzuki, Ren ^{[1
,4
]}

Tashima, Toshio ^{[4
]}

Fujii, Ken-ichi ^{[3
,4
]}

Masuda, Katsuyoshi ^{[4
]}

Awazu, Kunio ^{[1
,4
]}

Toyoda, Michisato ^{[2
,4
]}

Naito, Yasuhide ^{[4
]}

机构：

[1] Osaka Univ, Grad Sch Engn, Suita, Osaka 565, Japan

[2] Osaka Univ, Grad Sch Sci, Suita, Osaka 565, Japan

[3] Osaka Inst Technol, Osaka, Japan

[4] Japan Sci & Technol Agcy, CREST, Tokyo, Japan

来源：

APPLIED SURFACE SCIENCE | 2008年 / 255卷 / 04期

关键词：

Imaging mass spectrometry; Stigmatic mode; MALDI; Multi-turn TOF;

D O I：

10.1016/j.apsusc.2008.05.058

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

A novel design concept and the status of development of a matrix-assisted laser desorption/ionization (MALDI) imaging mass spectrometer in the stigmatic mode utilizing a perfect focusing property of a multi-turn time-of-flight (TOF) ion optics is reported. A multi-turn TOF ion optics consisting of four toroidal electric sectors has ideal features for a mass analyzer of stigmatic imaging mass spectrometry. The constructed multi-turn TOF mass analyzer, MULTUM-IMG, demonstrated a strong focusing property allowing MALDI-generated ions to revolve in a closed orbit for hundreds turns. Images of ion ensembles extracted from matrix surfaces patterned by several sizes of thin grid meshes (100-1000 lines/in.) or from arrays of small pigment dots (approximately 30 mu m in diameter) fabricated on Si plates were examined by attaching an imaging detector to a MALDI ion source directly, and were found to retain the original spatially distributing patterns of the samples. Images of a patterned ion ensemble after multiple revolutions in MULTUM-IMG were examined, and were found to retain the images at the entrance of the multi-turn TOF section. The possibility of the novel stigmatic MALDI TOF imaging mass spectrometer has been proven by examining the building blocks. (C) 2008 Elsevier B.V. All rights reserved.

引用

页码：1257 / 1263

页数：7

共 14 条

[1] High-resolution MALDI imaging mass spectrometry allows localization of peptide distributions at cellular length scales in pituitary tissue sections [J].