MicroRNA-200a Locally Attenuates Progesterone Signaling in the Cervix, Preventing Embryo Implantation

被引:52
作者
Haraguchi, Hirofumi [1 ]
Saito-Fujita, Tomoko [1 ]
Hirota, Yasushi [1 ,4 ]
Egashira, Mahiro [1 ,3 ]
Matsumoto, Leona [1 ]
Matsuo, Mitsunori [1 ]
Hiraoka, Takehiro [1 ]
Koga, Kaori [1 ]
Yamauchi, Naoko [2 ]
Fukayama, Masashi [2 ]
Bartos, Amanda [5 ]
Cha, Jeeyeon [5 ]
Dey, Sudhansu K. [5 ]
Fujii, Tomoyuki [1 ]
Osuga, Yutaka [1 ]
机构
[1] Univ Tokyo, Grad Sch Med, Dept Obstet & Gynecol, Tokyo 1138655, Japan
[2] Univ Tokyo, Grad Sch Med, Dept Pathol, Tokyo 1138655, Japan
[3] Univ Tokyo, Grad Sch Agr & Life Sci, Dept Vet Med Sci, Tokyo 1138655, Japan
[4] Japan Sci & Technol Agcy, Precursory Res Embryon Sci & Technol, Kawaguchi, Saitama 3320012, Japan
[5] Cincinnati Childrens Res Fdn, Perinatal Inst, Div Reprod Sci, Cincinnati, OH 45229 USA
基金
美国国家卫生研究院; 日本学术振兴会;
关键词
UTERINE RECEPTIVITY; RECEPTOR; PREGNANCY; DIFFERENTIATION; PROLIFERATION; ASSOCIATION; MECHANISMS; BINDING; FKBP52; CELLS;
D O I
10.1210/me.2014-1097
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Although cervical pregnancy and placenta previa, in which the embryo and placenta embed in or adjacent to the cervix, are life-threatening complications that result in massive bleeding and poor pregnancy outcomes in women, the incidence of these aberrant conditions is uncommon. We hypothesized that a local molecular mechanism is normally in place to prevent embryo implantation in the cervix. The ovarian hormones progesterone (P-4) and estrogen differentially direct differentiation and proliferation of endometrial cells, which confers the receptive state for implantation: P-4 dominance causes differentiation of the luminal epithelium but increases stromal cell proliferation in preparation of the uterus for implantation. In search for the cause of cervical nonresponsiveness to implantation, we found that the statuses of cell proliferation and differentiation between the uterus and cervix during early pregnancy are remarkably disparate under identical endocrine milieu in both mice and humans. We also found that cervical levels of progesterone receptor (PR) protein are low compared with uterine levels during this period, and the low PR protein levels are attributed to elevated levels of microRNA(miR)-200a in the cervix. These changes were associated with up-regulation of the P-4-metabolizing enzyme 20 alpha-hydroxysteroid dehydrogenase (200 alpha-HSD) and down-regulation of its transcriptional repressor signal transducer and activator of transcription 5 in the cervix. The results provide evidence that elevated levels of miR-200a lead to down-regulation of P-4-PR signaling and up-regulation of (200 alpha-HSD) in the cervix, rendering it nonresponsive to implantation. These findings may point toward not only the physiological but also the pathological basis of the cervical milieu in embryo implantation.
引用
收藏
页码:1108 / 1117
页数:10
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