Development of microsatellite markers in black locust (Robinia pseudoacacia) using a dual-supression-PCR technique

被引：142

作者：

Lian, C ^{[1
]}

Hogetsu, T ^{[1
]}

机构：

[1] Univ Tokyo, Asian Nat Environm Sci Ctr, Tokyo 1880002, Japan

来源：

MOLECULAR ECOLOGY NOTES | 2002年 / 2卷 / 03期

关键词：

(AC)(10) primer; black locust; dual-suppression-PCR technique; microsatellite; Robinia pseudoacacia;

D O I：

10.1046/j.1471-8278.2002.00213.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Black locust (Robinia pseudoacacia) is an economically and ecologically important tree species in the world. We isolated seven polymorphic microsatellite loci from R. pseudoacacia using a dual-suppression-PCR technique. These loci provided microsatellite markers with high polymorphism ranging from three to 12 alleles per locus and expected heterozygosity between 0.538 and 0.944. The markers are now available for more detailed investigation of population genetic structure and pollen and seed dispersal.

引用

页码：211 / 213

页数：3

共 6 条

[1] Dini-Papanastasi O, 2000, SILVAE GENET, V49, P37
[2] A simple method for developing microsatellite markers using amplified fragments of inter-simple sequence repeat (ISSR)
Lian, CL
Zhou, ZH
Hogetsu, T
[J]. JOURNAL OF PLANT RESEARCH, 2001, 114 (1115) : 381 - 385
[3] Statistical confidence for likelihood-based paternity inference in natural populations
Marshall, TC
Slate, J
Kruuk, LEB
Pemberton, JM
[J]. MOLECULAR ECOLOGY, 1998, 7 (05) : 639 - 655
[4] AN IMPROVED PCR METHOD FOR WALKING IN UNCLONED GENOMIC DNA
SIEBERT, PD
CHENCHIK, A
KELLOGG, DE
LUKYANOV, KA
LUKYANOV, SA
[J]. NUCLEIC ACIDS RESEARCH, 1995, 23 (06) : 1087 - 1088
[5] ALLOZYME VARIATION IN BLACK LOCUST (ROBINIA-PSEUDOACACIA)
SURLES, SE
HAMRICK, JL
BONGARTEN, BC
[J]. CANADIAN JOURNAL OF FOREST RESEARCH-REVUE CANADIENNE DE RECHERCHE FORESTIERE, 1989, 19 (04): : 471 - 479
[6] Analysis of genetic structure of a Suillus grevillei population in a Larix kaempferi stand by polymorphism of inter-simple sequence repeat (ISSR)
Zhou, ZH
Miwa, M
Hogetsu, T
[J]. NEW PHYTOLOGIST, 1999, 144 (01) : 55 - 63

← 1 →