Biological activity of five antibacterial flavonoids from Combretum erythrophyllum (Combretaceae)

被引:203
作者
Martini, ND [1 ]
Katerere, DRP [1 ]
Eloff, JN [1 ]
机构
[1] Univ Pretoria, Dept Paraclin Sci, Programme Phytomed, ZA-0002 Pretoria, South Africa
基金
新加坡国家研究基金会;
关键词
Combretum erythrophyllum; antimicrobial; antioxidant; anti-inflammatory; toxicity; flavonoids;
D O I
10.1016/j.jep.2004.02.030
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Preliminary studies with Combretum erythrophyllum showed antimicrobial activity against Gram-positive and Gram-negative bacteria. Seven antibacterial flavonoids were subsequently isolated by bioassay-guided fractionation, i.e. apigenin; genkwanin; 5-hydroxy-7,4'-dimethoxyflavone, rhamnocitrin; kaempferol; quercetin-5,3'-dimethylether; rhamnazin. All compounds had good activity against Vibrio cholerae and Enterococcus faecalis, with MIC values in the range of 25-50 mug/ml. Rhamnocitrin and quercetin-5,3'-dimethylether also inhibited Micrococcus luteus and Shigella sonei at 25 mug/ml. With the exception of 5-hydroxy-7,4'-dimethoxy-flavone the flavonoids were not toxic towards human lymphocytes. This compound is potentially toxic to human cells and exhibited the poorest antioxidant activity whereas rhamnocitrin and rhamnazin exhibited strong antioxidant activity. Genkwanin; rhamnocitrin; quercetin-5,3'-dimethylether; rhamnazin had a higher anti-inflammatory activity than the positive control mefenamic acid. Although these flavonoids are known, this is the first report of biological activity with several of these compounds. (C) 2004 Elsevier Ireland Ltd. All rights reserved.
引用
收藏
页码:207 / 212
页数:6
相关论文
共 28 条
[11]   A SURVEY OF ANTIFUNGAL COMPOUNDS FROM HIGHER-PLANTS, 1982-1993 [J].
GRAYER, RJ ;
HARBORNE, JB .
PHYTOCHEMISTRY, 1994, 37 (01) :19-42
[12]  
HUTCHINGS S, 1996, ZULU MEDICINAL PLANT
[13]  
IBRAHIM RK, 2000, FLAVONOIDS
[14]   Antimicrobial activity of pentacyclic triterpenes isolated from African Combretaceae [J].
Katerere, DR ;
Gray, AI ;
Nash, RJ ;
Waigh, RD .
PHYTOCHEMISTRY, 2003, 63 (01) :81-88
[15]   ISOLATION AND ANTI-MICROBIAL ACTIVITY OF THE PHYTOALEXIN 6-METHOXYMELLEIN FROM CULTURED CARROT CELLS [J].
KUROSAKI, F ;
NISHI, A .
PHYTOCHEMISTRY, 1983, 22 (03) :669-672
[16]  
López-Lázaro M, 2000, Z NATURFORSCH C, V55, P898
[17]   A glycosyl analogue of diacylglycerol and other antiinflammatory constituents from Inula viscosa [J].
Máñez, S ;
Recio, MD ;
Gil, I ;
Gómez, C ;
Giner, RM ;
Waterman, PG ;
Ríos, JL .
JOURNAL OF NATURAL PRODUCTS, 1999, 62 (04) :601-604
[18]   The preliminary isolation of several antibacterial compounds from Combretum erythrophyllum (Combretaceae) [J].
Martini, N ;
Eloff, JN .
JOURNAL OF ETHNOPHARMACOLOGY, 1998, 62 (03) :255-263
[19]  
MARTINI ND, 2004, IN PRESS S AFRICAN J
[20]  
MARTINI ND, 2002, THESIS U PRETORIA S