Human metallothionein enhanced tolerance and biosorption of cadmium when expressed in Saccharomyces cerevisiae

被引：1

作者：

Liang, Bo ^{[1
]}

Li, Min ^{[1
]}

Shang, Jie ^{[1
]}

机构：

[1] E China Inst Technol, Fundamental Sci Radioact Geol & Explorat Technol, Nanchang, Peoples R China

来源：

MATERIALS, TRANSPORTATION AND ENVIRONMENTAL ENGINEERING, PTS 1 AND 2 | 2013年 / 779-780卷

基金：

美国国家科学基金会;

关键词：

biosorption; metals; Saccharomyces cerevisiae; metallothionein; RECOMBINANT; METALS; COLI;

D O I：

10.4028/www.scientific.net/AMR.779-780.195

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

The recombinant Saccharomyces cerevisiae expressing human hepatic metallothionein (MT) was constructed for biosorption of cadmium (II). The gene sequence of mt was modified for codon preference of S. cerevisiae and synthesized using chemical method. The maximal biosorption capability of cadmium compounds Cd2+ of the recombinant increased more than 25.8% compared with the control. For MT-expressed recombinant strains, a rapid adsorption occurred within the first 30 min with a significant level of Cd2+ (55.75mg/g). For comparison, S. cerevisiae cells reach its maximal Cd2+ adsorption capacity (45.02 mg/g) until 2h. Furthermore, recombinant strain were able to withstand the toxicity of Cd2+ and grow. The results indicated that recombinant should be useful in enhancement the tolerance and biosorption of cadmium in practice.

引用

页码：195 / +

页数：3

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