The Histone Chaperone FACT Induces Cas9 Multi-turnover Behavior and Modifies Genome Manipulation in Human Cells

被引:27
作者
Wang, Alan S. [1 ,2 ]
Chen, Leo C. [1 ,2 ]
Wu, R. Alex [3 ,4 ]
Hao, Yvonne [1 ]
McSwiggen, David T. [1 ,5 ]
Heckert, Alec B. [1 ,5 ]
Richardson, Christopher D. [1 ,2 ]
Gowen, Benjamin G. [1 ,2 ]
Kazane, Katelynn R. [1 ,2 ]
Vu, Jonathan T. [1 ,2 ]
Wyman, Stacia K. [1 ,2 ]
Shin, Jiyung J. [1 ,2 ]
Darzacq, Xavier [1 ,5 ]
Walter, Johannes C. [3 ,4 ]
Corn, Jacob E. [1 ,2 ,6 ]
机构
[1] Univ Calif Berkeley, Dept Mol & Cell Biol, Berkeley, CA 94720 USA
[2] Univ Calif Berkeley, Innovat Genom Inst, Berkeley, CA 94720 USA
[3] Harvard Med Sch, Dept Biol Chem & Mol Pharmacol, Boston, MA 02115 USA
[4] Harvard Med Sch, Howard Hughes Med Inst, Dept Biol Chem & Mol Pharmacol, Boston, MA 02115 USA
[5] Univ Calif Berkeley, Calif Inst Regenerat Med Ctr Excellence, Berkeley, CA 94720 USA
[6] Swiss Fed Inst Technol, Dept Biol, CH-8093 Zurich, Switzerland
基金
美国国家科学基金会;
关键词
RNA-POLYMERASE-II; HOMOLOGOUS RECOMBINATION; DNA-REPLICATION; CHROMATIN; CRISPR-CAS9; NUCLEOSOME; COMPLEX; TRANSCRIPTION; DYNAMICS; REPAIR;
D O I
10.1016/j.molcel.2020.06.014
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Cas9 is a prokaryotic RNA-guided DNA endonuclease that binds substrates tightly in vitro but turns over rapidly when used to manipulate genomes in eukaryotic cells. Little is known about the factors responsible for dislodging Cas9 or how they influence genome engineering. Unbiased detection through proximity labeling of transient protein interactions in cell-free Xenopus laevis egg extract identified the dimeric histone chaperone facilitates chromatin transcription (FACT) as an interactor of substrate-bound Cas9. FACT is both necessary and sufficient to displace dCas9, and FACT immunodepletion converts Cas9's activity from multi-turnover to single turnover. In human cells, FACT depletion extends dCas9 residence times, delays genome editing, and alters the balance between indel formation and homology-directed repair. FACT knockdown also increases epigenetic marking by dCas9-based transcriptional effectors with a concomitant enhancement of transcriptional modulation. FACT thus shapes the intrinsic cellular response to Cas9-based genome manipulation most likely by determining Cas9 residence times.
引用
收藏
页码:221 / +
页数:18
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