Identification of defense-related genes in tobacco responding to black shank disease

被引：22

作者：

Chacon, Osmany ^{[1
,2
]}

Hernandez, Ingrid ^{[1
]}

Portieles, Roxana ^{[1
]}

Lopez, Yunior ^{[1
]}

Pujol, Merardo ^{[1
]}

Borras-Hidalgo, Orlando ^{[1
]}

机构：

[1] Ctr Genet Engn & Biotechnol, Lab Plant Funct Genom, Havana 10600, Cuba

[2] Tobacco Res Inst, Havana, Cuba

来源：

PLANT SCIENCE | 2009年 / 177卷 / 03期

关键词：

Nicotiana megalosiphon; Phytophthora parasitica var. nicotianae; Suppression subtractive hybridization; Plant resistance; GLUTATHIONE-S-TRANSFERASES; CATHEPSIN-B; RESISTANCE; INVOLVEMENT; MECHANISMS; INDUCTION; INFECTION; PATHOGEN; ENZYMES; PROTEIN;

D O I：

10.1016/j.plantsci.2009.05.009

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In order to identify tobacco (Nicotiana megalosiphon) genes involved in broad-spectrum resistance to tobacco black shank (Phytophthora parasitica var. nicotianae), suppression subtractive hybridization (SSH) was used to generate a cDNA from transcripts that are differentially expressed during an incompatible interaction. Forty-eight differentially expressed genes were selected, sequenced and analyzed. The cDNA collection comprised a repertoire of genes associated with various processes. Real-time PCR analysis of a subset of these genes confirmed the differential expression patterns between the compatible and incompatible interaction. The experiments demonstrated for the first time that hrs203J gene and RING finger protein gene exhibited strong induction during several incompatible interactions. Also, these genes were found not to be induced in compatible interactions. The set of differentially expressed tobacco genes associated to resistance could be exploited in strategies to develop durable resistance in cultivated tobacco plants. (C) 2009 Elsevier Ireland Ltd. All rights reserved.

引用

页码：175 / 180

页数：6

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