Development of a Molecular Imprinting-Based Surface Plasmon Resonance Biosensor for Rapid and Sensitive Detection of Staphylococcus aureus Alpha Hemolysin From Human Serum

被引:21
作者
Andersson, Tilde [1 ]
Blackberg, Anna [1 ,2 ]
Lood, Rolf [1 ]
Erturk Bergdahl, Gizem [1 ]
机构
[1] Lund Univ, Dept Clin Sci Lund, Div Infect Med, Lund, Sweden
[2] Skane Univ Hosp, Dept Infect Med, Lund, Sweden
来源
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY | 2020年 / 10卷
关键词
sepsis; Staphylococcus aureus; alpha hemolysin; molecular imprinting; SPR; ANTIMICROBIAL SUSCEPTIBILITY; LATIN-AMERICA; UNITED-STATES; SURVEILLANCE; INFECTIONS; ANTIBODIES; FREQUENCY; BACTERIAL; CANADA;
D O I
10.3389/fcimb.2020.571578
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Stapylococcus aureus is a common infectious agent in e.g. sepsis, associated with both high mortality rates and severe long-term effects. The cytolytic protein alpha-hemolysin has repeatedly been shown to enhance the virulence of S. aureus. Combined with an unhindered spread of multi drug-resistant strains, this has triggered research into novel anti virulence (i.e. anti alpha-hemolysin) drugs. Their functionality will depend on our ability to identify infections that might be alleviated by such. We therefore saw a need for detection methods that could identify individuals suffering from S. aureus infections where alpha-hemolysin was a major determinant. Molecular imprinted polymers were subsequently prepared on gold coated sensor chips. Used in combination with a surface plasmon resonance biosensor, alpha-hemolysin could therethrough be quantified from septic blood samples (n = 9), without pre-culturing of the infectious agent. The biosensor recognized alpha-hemolysin with high affinity (K-D = 2.75 x 10(-7) M) and demonstrated a statistically significant difference (p < 0.0001) between the alpha-hemolysin response and potential sample contaminants. The detection scheme proved equally good, or better, when compared to antibody-based detection methods. This novel detection scheme constitutes a more rapid, economical, and user-friendly alternative to many methods currently in use. Heightening both reproducibility and sensitivity, molecular imprinting in combination with surface plasmon resonance (SPR)-technology could be a versatile new tool in clinical- and research-settings alike.
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页数:8
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