Determination of Belotecan in the Plasma, Bile, and Urine of Rats by High-Performance Liquid Chromatography with Fluorescence Detection and Its Application to a Pharmacokinetic Study

被引：1

作者：

Jin, Qing-Ri ^{[2
,3
]}

Kim, In-Wha ^{[2
,3
]}

Choi, Min-Koo ^{[2
,3
]}

Shim, Won-Sik ^{[2
,3
]}

Tian, Guang-Yan

Lee, Soohyun ^{[2
,3
]}

Lee, Jonghwa

Kim, Dae-Duk

Chung, Suk-Jae

Shim, Chang-Koo ^{[1
,2
,3
]}

机构：

[1] Seoul Natl Univ, Natl Res Lab Transporters, Targeted Drug Design & Res Inst Pharmaceut Sci, Coll Pharm, Seoul, South Korea

[2] Seoul Natl Univ, Pharmaceut Sci Res Inst, Coll Pharm, Seoul, South Korea

[3] Seoul Natl Univ, Dept Pharmaceut, Coll Pharm, Seoul, South Korea

来源：

ANALYTICAL LETTERS | 2009年 / 42卷 / 01期

关键词：

Belotecan; bile; HPLC; plasma; rat; urine; SIMULTANEOUS QUANTITATION; CARBOXYLATE FORMS; METABOLITE SN-38; CAMPTOTHECIN; LACTONE; TOPOTECAN; IRINOTECAN; CKD-602; ANALOGS; CPT-11;

D O I：

10.1080/00032710802568572

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A simple and reliable high-performance liquid chromatographic (HPLC) method was developed for the determination of belotecan in the plasma, urine, and bile samples of rats. Belotecan was analyzed with HPLC using a C18 column with fluorescence detector. A mixture of acetonitrile-0.1M potassium phosphate buffer at pH 2.4 (25:75, v/v) and 0.2% trifluoroacetic acid was used as the mobile phase. The lower limits of quantitation (LOQ) were 5 ng mL(-1) for the plasma and 5 mu g mL(-1) for the urine and bile samples. The method has been readily applied for the routine pharmacokinetic study of belotecan in small laboratory animals.

引用

页码：68 / 83

页数：16

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