The influence of cytokines on the integrity of the blood-brain barrier in vitro

被引：550

作者：

deVries, HE ^{[1
]}

BlomRoosemalen, MCM ^{[1
]}

vanOosten, M ^{[1
]}

deBoer, AG ^{[1
]}

vanBerkel, TJC ^{[1
]}

Breimer, DD ^{[1
]}

Kuiper, J ^{[1
]}

机构：

[1] LEIDEN UNIV, AMSTERDAM CTR DRUG RES, DIV BIOPHARMACEUT, SYLVIUS LABS, 2300 RA LEIDEN, NETHERLANDS

来源：

JOURNAL OF NEUROIMMUNOLOGY | 1996年 / 64卷 / 01期

关键词：

blood-brain barrier; cytokines; cerebral endothelial cells; inflammation;

D O I：

10.1016/0165-5728(95)00148-4

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

The effects of the cytokines tumour necrosis factor-alpha (TNF-alpha, interleukin (IL)-1 beta and IL-6 on the permeability of monolayers of rat cerebral endothelial cells (RCEC) were investigated to assess potential changes in the integrity of the blood-brain barrier (BBB). RCEC were cultured to tight monolayers with a trans endothelial electrical resistance (TEER) of 100-150 Omega . cm(2) on polycarbonate filters. Exposure of the RCEC to TNF-alpha, IL-1 beta and IL-6 induced a decline in the TEER, which could be completely abolished by 1 mu M of indomethacin, a cyclooxygenase inhibitor. In addition, the effect of IL-1 beta on TEER across monolayers of RCEC could be completely inhibited by IL-1 receptor antagonist. In conclusion, cytokines induce a disruption of the BBB in vitro. In this process, cyclooxygenase activation within the endothelial cells seems to play a key role.

引用

页码：37 / 43

页数：7

共 39 条

[1] CHARACTERIZATION OF RECEPTORS FOR HUMAN-TUMOR NECROSIS FACTOR AND THEIR REGULATION BY GAMMA-INTERFERON [J].