The dynamics of gene expression, lipid composition and DNA methylation reprogramming are different during in vitro maturation of pig oocytes obtained from prepubertal gilts and cycling sows

被引：7

作者：

Braga, Thiago Felipe ^{[1
,2
]}

Ferreira Silva, Thainara Christie ^{[2
,3
]}

Marques, Mariana Groke ^{[4
]}

de Souza, Andressa Pereira ^{[5
]}

Albring, Daniela ^{[6
]}

Silva, Luciano Paulino ^{[2
]}

Caetano, Alexandre Rodrigues ^{[2
]}

Nunes Dode, Margot Alves ^{[2
]}

Franco, Mauricio Machaim ^{[2
,3
,7
]}

机构：

[1] Univ Brasilia UnB, Brasilia, DF, Brazil

[2] Embrapa Recursos Genet & Biotecnol, Brasilia, DF, Brazil

[3] Univ Fed Uberlandia, Fac Med Vet, Uberlandia, MG, Brazil

[4] Embrapa Suinos & Ayes, Concordia, Brazil

[5] Univ Estado Santa Catarina UDE, Florianopolis, SC, Brazil

[6] Univ Contestado UnC, Mafra, Brazil

[7] Univ Fed Uberlandia, Inst Genet & Bioquim, Uberlandia, MG, Brazil

来源：

REPRODUCTION IN DOMESTIC ANIMALS | 2019年 / 54卷 / 09期

关键词：

Epigenetics; gene expression; molecular markers; oocyte competence; pig; EARLY EMBRYO DEVELOPMENT; PORCINE OOCYTES; DEVELOPMENTAL COMPETENCE; MAMMALIAN OOCYTES; X-INACTIVATION; SHEEP OOCYTES; FERTILIZATION; METABOLISM; APOPTOSIS; GENOME;

D O I：

10.1111/rda.13501

中图分类号：

S8 [畜牧、动物医学、狩猎、蚕、蜂];

学科分类号：

0905 ;

摘要：

This study aimed to characterize the gene expression, lipid composition and DNA methylation reprogramming during in vitro maturation (IVM) of pig oocytes with different developmental competencies. We used prepubertal gilts and cycling sows as a model to obtain oocytes with different levels of competency. We found that genes involved in lipid metabolism, SLC27A4, CPT2 and PLIN2, and DNA methylation, DNMT3A, TET1 and TET3, possessed altered transcript expression levels during IVM. Specifically, SLC27A4 mRNA (p = 0.05) increased in oocytes from cycling females, whereas CPT2 (p = 0.05), PLIN2 (p = 0.02) and DNMT3A (p = 0.02) increased in oocytes from prepubertal females during IVM. Additionally, TET3 mRNA increased during IVM in oocytes from prepubertal (p = 0.0005) and cycling females (p = 0.02). The TET1 transcript decreased (p = 0.05) during IVM in oocytes from cycling sows. Regarding lipid composition, mass spectrometry revealed a cluster of ions, with molecular masses higher than m/z 700, which comprises a group of complex phospholipids, was identified in all groups of oocytes, except in those from prepubertal gilts. With respect to DNA methylation reprogramming, it was noted that the less competent oocytes were not able to reprogramme the XIST gene during IVM. We conclude that the maternal mRNA store, lipid composition and epigenetic reprogramming are still being established during maturation and are related to oocyte competence. In addition, we propose that the methylation pattern of the XIST may be used as molecular marker for oocyte competence in pigs.

引用

页码：1217 / 1229

页数：13

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