Complete Proteome of a Quinolone-Resistant Salmonella Typhimurium Phage Type DT104B Clinical Strain

被引:13
作者
Correia, Susana [1 ,2 ,3 ,4 ]
Nunes-Miranda, Julio D. [1 ,2 ]
Pinto, Luis [1 ,2 ,3 ,4 ]
Santos, Hugo M. [5 ]
de Toro, Maria [6 ,7 ]
Saenz, Yolanda [8 ]
Torres, Carmen [8 ,9 ]
Capelo, Jose Luis [5 ]
Poeta, Patricia [3 ,4 ]
Igrejas, Gilberto [1 ,2 ]
机构
[1] Univ Tras Os Montes & Alto Douro, Inst Biotechnol & Bioengn, Ctr Genom & Biotechnol, P-5001801 Vila Real, Portugal
[2] Univ Tras Os Montes & Alto Douro, Dept Genet & Biotechnol, P-5001801 Vila Real, Portugal
[3] Univ Tras Os Montes & Alto Douro, Ctr Studies Anim & Vet Sci, P-5001801 Vila Real, Portugal
[4] Univ Tras Os Montes & Alto Douro, Dept Vet Sci, P-5001801 Vila Real, Portugal
[5] Univ Nova Lisboa, Fac Sci & Technol, Dept Chem, BIOSCOPE Grp,REQUIMTE CQFB, P-2829516 Monte De Caparica, Portugal
[6] Univ Cantabria, Dept Biol Mol, Santander 39011, Spain
[7] Inst Biomed & Biotecnol Cantabria IBBTEC UC SODER, Santander 39011, Spain
[8] Ctr Invest Biomed La Rioja, Area Microbiol Mol, Logrono 26006, La Rioja, Spain
[9] Univ La Rioja, Area Bioquim & Biol Mol, Logrono 26006, La Rioja, Spain
关键词
Salmonella enterica serovar Typhimurium; DT104B; SL1344; proteome; aminoglycoside 6-N-acetyltransferase type Ib-cr4; ENTERICA SEROVAR TYPHIMURIUM; RAW; 264.7; MACROPHAGES; 2-DIMENSIONAL ELECTROPHORESIS; OUTER-MEMBRANE; ANTIBIOTIC-RESISTANCE; MASS SPECTROMETRY; PROTEINS; GENE; IDENTIFICATION; ENTERITIDIS;
D O I
10.3390/ijms150814191
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Salmonellosis is one of the most common and widely distributed foodborne diseases. The emergence of Salmonella strains that are resistant to a variety of antimicrobials is a serious global public health concern. Salmonella enterica serovar Typhimurium definitive phage type 104 (DT104) is one of these emerging epidemic multidrug resistant strains. Here we collate information from the diverse and comprehensive range of experiments on Salmonella proteomes that have been published. We then present a new study of the proteome of the quinolone-resistant Se20 strain (phage type DT104B), recovered after ciprofloxacin treatment and compared it to the proteome of reference strain SL1344. A total of 186 and 219 protein spots were recovered from Se20 and SL1344 protein extracts, respectively, after two-dimensional gel electrophoresis. The signatures of 94% of the protein spots were successfully identified through matrix-assisted laser desorption/ionization mass spectrometry (MALDI-TOF MS). Three antimicrobial resistance related proteins, whose genes were previously detected by polymerase chain reaction (PCR), were identified in the clinical strain. The presence of these proteins, dihydropteroate synthase type-2 (sul2 gene), aminoglycoside resistance protein A (strA gene) and aminoglycoside 6'-N-acetyltransferase type Ib-cr4 (aac(6')-Ib-cr4 gene), was confirmed in the DT104B clinical strain. The aac(6')-Ib-cr4 gene is responsible for plasmid-mediated aminoglycoside and quinolone resistance. This is a preliminary analysis of the proteome of these two S. Typhimurium strains and further work is being developed to better understand how antimicrobial resistance is developing in this pathogen.
引用
收藏
页码:14191 / 14219
页数:29
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