Identification of heat shock cognate protein 70 gene (Alhsc70) of Apolygus lucorum and its expression in response to different temperature and pesticide stresses

被引：51

作者：

Sun, Yang ^{[1
,2
]}

Zhao, Jing ^{[1
]}

Sheng, Yang ^{[1
]}

Xiao, Ying-Fang ^{[3
]}

Zhang, Yong-Jun ^{[2
]}

Bai, Li-Xin ^{[1
]}

Tan, Yongan ^{[1
]}

Xiao, Liu-Bin ^{[1
]}

Xu, Guang-Chun ^{[1
]}

机构：

[1] Jiangsu Acad Agr Sci, Inst Plant Protect, Nanjing 210014, Jiangsu, Peoples R China

[2] Chinese Acad Agr Sci, Inst Plant Protect, Lab Biol Plant Dis & Insect Pests, Beijing 100193, Peoples R China

[3] Univ Florida, Mid Florida Res & Educ Ctr, Entomol & Nematol, Apopka, FL USA

来源：

INSECT SCIENCE | 2016年 / 23卷 / 01期

基金：

中国国家自然科学基金;

关键词：

Apolygus lucorum; expression files; extreme temperature; hsc70; pesticide; HSP70; GENE; MOLECULAR CHAPERONES; PTEROMALUS-PUPARUM; LARVAL DIAPAUSE; THERMAL-STRESS; SPIDER-MITE; MEYER-DUR; CLONING; HEAT-SHOCK-PROTEIN-70; HSP90;

D O I：

10.1111/1744-7917.12193

中图分类号：

Q96 [昆虫学];

学科分类号：

摘要：

Heat shock cognate protein 70 (Hsc70) is a very important stress-resistance protein of insects against environmental stresses. We employed fluorescent real-time quantitative polymerase chain reaction and Western-blot techniques to analyze the transcriptional and translational expression profiles of AlHSC70 under extreme temperature (4 degrees C and 40 degrees C) or 4 pesticide stresses in Apolygus lucorum. The results showed that the expression of AlHSC70 were significantly induced by cyhalothrin or extremely high temperature (40 degrees C) in both transcriptional and translational levels (P < 0.05), while the transcriptional and translational level of AlHSC70 decreased significantly in treatments of chlorpyrifos or extreme cold temperature (4 degrees C) (P < 0.05). Moreover, after Apolygus lucorum treated by imidacloprid or emamectin benzoate, the expression of AlHSC70 was only up-regulated significantly at the transcriptional level (P < 0.05), although obviously up-regulated at the translational level of AlHSC70. Therefore, this study confirmed that the Alhsc70 gene played important roles in response to both temperature and pesticide stresses, especially for cyhalothrin or extremely high temperature (40 degrees C). In addition, the significant polynomial regression correlations between temperature and the Alhsc70 expression level were shown in all the nymph and adult stages (P < 0.01), indicating temperature was an important factor to affect the relative expression of Alhsc70.

引用

页码：37 / 49

页数：13

共 39 条

[1] Survival and Hsp70 Gene Expression in Plutella xylostella and Its Larval Parasitoid Diadegma insulare Varied between Slowly Ramping and Abrupt Extreme Temperature Regimes [J].

Bahar, Md Habibullah ;

Hegedus, Dwayne ;

Soroka, Juliana ;

Coutu, Cathy ;

Bekkaoui, Diana ;

Dosdall, Lloyd .

PLOS ONE, 2013, 8 (09)

[2]

Chen B, 2006, J BIOCHEM MOL BIOL, V39, P749

[3]

Daugaard M, 2007, FEBS LETT, V581, P3702, DOI [10.1016/j.febslet.2007.05.039, 10.1016/j.febsiet.2007.05.039]

[4]

Denlinger David L., 2001, P155, DOI 10.1016/B978-044450608-5/50045-3

[5]

FAO, 1980, 3 FAO, P25

[6] Heat-shock proteins, molecular chaperones, and the stress response: Evolutionary and ecological physiology [J].

Feder, ME ;

Hofmann, GE .

ANNUAL REVIEW OF PHYSIOLOGY, 1999, 61 :243-282

[7]

Feng HZ, 2010, J INSECT SCI, V10, DOI 10.1673/031.010.11201

[8] Thermodynamic stability of the C-terminal domain of the human inducible heat shock protein 70 [J].

Fuertes, MA ;

Pérez, JM ;

Soto, M ;

Menéndez, M ;

Alonso, C .

BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS, 2004, 1699 (1-2) :45-56

[9] Cognate Hsp70 gene is induced during deep larval diapause in the moth Sesamia nonagrioides [J].

Gkouvitsas, T. ;

Kontogiannatos, D. ;

Kourti, A. .

INSECT MOLECULAR BIOLOGY, 2009, 18 (02) :253-264

[10] Protein folding - Molecular chaperones in the cytosol: from nascent chain to folded protein [J].

Hartl, FU ;

Hayer-Hartl, M .

SCIENCE, 2002, 295 (5561) :1852-1858

← 1 2 3 4 →